Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780134433769
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman
Publisher: PEARSON
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Textbook Question
Chapter 14.4, Problem 3CC
Describe how a polypeptide to be secreted is transported to the endomembrane system.
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True or False?
a.____If a restriction enzyme recognizes the restriction site, 5’AACGTT3’, and the enzyme cuts between the second A and the C, this will produce a “sticky end,” which is useful for cloning into a plasmid vector.
b.____The DNA molecules will travel through the agar toward the negative end of the gel rig (black wires) during electrophoresis.
c.____CRISPR was one of the first methods discovered in the 1970s for reliably amplifying gene products so that they can be sequenced and studied.
a) Polymerase Chain Reaction is a way that scientists amplify (or create billions of copies of) DNA in vitro (in a tube.)
b) Compare PCR with DNA Replication in the cell. Circle the components that are required for PCR. For the parts that are not circled, list them below and write an explanation for why they are no longer needed when DNA replication of a target gene is done in vitro.
DNA Gyrase (or Topoisomerase)
DNA polymerase
Helicase
SSBPs
Ligase
dNTPs
Primers
Sliding Clamp
Replisome
Draw the major product of this reaction. Ignore inorganic byproducts.
O
1. NaOCH2CH3, 25°C
2. PhCH2Br (1 equiv)
Chapter 14 Solutions
Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
Ch. 14.1 - MAKE CONNECTIONS In a research article about...Ch. 14.1 - What polypeptide product would you expect from a...Ch. 14.1 - DRAW IT The template strand of a gene contains the...Ch. 14.2 - What is a promoter? Is it located at the upstream...Ch. 14.2 - What enables RNA polymerase to start transcribing...Ch. 14.2 - WHAT IF? Suppose X-rays caused a sequence change...Ch. 14.3 - Given that there are about 20,000 human genes, how...Ch. 14.3 - How is RNA splicing similar to how you would watch...Ch. 14.3 - WHAT IF? What would be the effect of treating...Ch. 14.4 - What two processes ensure that the correct amino...
Ch. 14.4 - Discuss the ways in which rRNA structure likely...Ch. 14.4 - Describe how a polypeptide to be secreted is...Ch. 14.4 - WHAT IF? DRAW IT Draw a tRNA with the anticodon...Ch. 14.5 - What happens when one nucleotide pair is lost from...Ch. 14.5 - Prob. 2CCCh. 14.5 - WHAT IF? DRAW IT The template strand of a gene...Ch. 14 - In eukaryotic cells, transcription cannot begin...Ch. 14 - Prob. 2TYUCh. 14 - The anticodon of a particular tRNA molecule is A....Ch. 14 - Prob. 4TYUCh. 14 - Which component is not directly involved in...Ch. 14 - Prob. 6TYUCh. 14 - Prob. 7TYUCh. 14 - Prob. 8TYUCh. 14 - Fill in the following table: Type of RNA Functions...Ch. 14 - SCIENTIFIC INQUIRY Knowing that the genetic code...Ch. 14 - Prob. 11TYUCh. 14 - FOCUS ON INFORMATION Evolution accounts for the...Ch. 14 - SYNTHESIZE YOUR KNOWLEDGE Some mutations result in...
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