Concept explainers
a.
To determine: Whether, any of the suspects can be excluded as the one who committed the crime based on the results of the testing, or not.
Introduction: The genetic material of an individual is packed in the form of DNA. The evidence collected by the police is a small bloodstain from which the DNA is extracted and the fragments are separated with the help of electrophoresis. The separated fragments are transferred to a membrane where they are probed with radioactive DNA.
b.
To determine: The suspect which can be excluded as the one who committed the crime with a plausible reason.
Introduction: The genetic material of an individual is packed in the form of DNA. The evidence collected by the police is a small bloodstain from which the DNA is extracted and the fragments are separated with the help of electrophoresis. The separated fragments are transferred to a membrane where they are probed with radioactive DNA.
c.
To determine: Whether the pattern from the evidence is consistent with the band pattern of one of the suspects, or not and also determine the suspect with which it is consistent.
Introduction: The genetic material of an individual is packed in the form of DNA. The evidence collected by the police is a small bloodstain from which the DNA is extracted and the fragments are separated with the help of electrophoresis. The separated fragments are transferred to a membrane where they are probed with radioactive DNA.
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Human Heredity: Principles and Issues (MindTap Course List)
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardDNA Profiles as Tools for Identification STRs are: a. used for DNA profiles b. repeated sequences present in the human genome c. highly variable in copy number d. all of these e. none of thesearrow_forwardDNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?arrow_forward
- Which technique used to manipulate genetic material results in a significant increase in DNA or RNA fragments? Group of answer choices heat shock treatment electroporation gel electrophoresis polymerase chain reactionarrow_forwardGel Electrophoresis is used often in forensics. Look at the following gel to the left. From the evidence DNA, which individual matches the DNA evidence left at the crime scene?arrow_forwardLoading dye stains the DNA molecules and thus help to visualize it under UV light apparatus. True Falsearrow_forward
- When a DNA sample is subjected to sequencing, the two strands must be separated. True Falsearrow_forwardYou are provided with coiled DNA and plasmid DNA that you subject to gel electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. Exact fragment sizes are not important.arrow_forwardBriefly explain the functions of the four procedures you learned about in this lab: DNA extraction PCR Gel electrophoresis DNA sequencing and analysisarrow_forward
- Why PCR needs the following: DNA template Primers DNA polymerase enzymes dNTPs Buffer Mg2+arrow_forwardRestriction enzymes bind to specific sequences of DNA to seal them together. True Falsearrow_forwardWhich of the following gels is the preferred choice for separation of DNA fragments? Agarose Polyacrylamide SDS PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) Agararrow_forward
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