Microbiology: A Laboratory Manual (11th Edition)
11th Edition
ISBN: 9780134098630
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 14, Problem 58E.7RQ
Summary Introduction
To explain: The role of restriction enzymes in developing an organism to produce a protein that is not normally made by the organism.
Introduction: Restriction enzymes are called molecular scissors that cleave the DNA into smaller fragments. These enzymes are called endonucleases. These enzymes are used to create DNA recombinants, to generate DNA profiles, to map the restriction sites on the plasmids, and to detect genetic defects.
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Transcription and Translation
1. What is the main function of transcription and translation? (2
marks)
2. How is transcription different in eukaryotic and prokaryotic
cells? (2 marks)
3. Explain the difference between pre-mRNA and post-transcript
mRNA. (2 marks)
4. What is the function of the following: (4 marks)
i. the cap
ii. spliceosome
iii. Poly A tail
iv. termination sequence
5. What are advantages to the wobble feature of the genetic
code? (2 marks)
6. Explain the difference between the: (3 marks)
i. A site & P site
ii. codon & anticodon
iii. gene expression and gene regulation
7. Explain how the stop codon allows for termination. (1 mark)
8. In your own words, summarize the process of translation. (2
marks)
In this activity you will research performance enhancers that affect the
endocrine system or nervous system. You will submit a 1 page paper on one
performance enhancer of your choice. Be sure to include:
the specific reason for use
the alleged results on improving performance
how it works
how it affect homeostasis and improves performance
any side-effects of this substance
Chapter 14 Solutions
Microbiology: A Laboratory Manual (11th Edition)
Ch. 14 - Prob. 56E.1RQCh. 14 - Prob. 56E.2RQCh. 14 - Prob. 56E.3RQCh. 14 - Why is plasmid DNA preferred for genetic...Ch. 14 - What are selectable markers, and why are they...Ch. 14 - Prob. 57E.3RQCh. 14 - Alcohol is considered to be a significant reagent...Ch. 14 - Prob. 57E.5RQCh. 14 - Prob. 57E.6RQCh. 14 - Prob. 57E.7RQ
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