Microbiology: A Laboratory Manual (11th Edition)
Microbiology: A Laboratory Manual (11th Edition)
11th Edition
ISBN: 9780134098630
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 14, Problem 58E.5RQ
Summary Introduction

To explain: The reason to add the restriction enzymes at last to the digestion mixture.

Introduction: The endonucleases are the enzymes, which are also called restriction enzymes or restriction endonucleases that are able to cleave the phosphodiester bonds of the DNA. These restriction endonucleases are able to cut the DNA at any specific ends. The use of restriction endonucleases has specific importance in the field of recombinant DNA technology.     

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