The reason to add the restriction enzymes at last to the digestion mixture. Introduction: The endonucleases are the enzymes, which are also called restriction enzymes or restriction endonucleases that are able to cleave the phosphodiester bonds of the DNA. These restriction endonucleases are able to cut the DNA at any specific ends. The use of restriction endonucleases has specific importance in the field of recombinant DNA technology.

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Chapter 14, Problem 58E.5RQ

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To explain: The reason to add the restriction enzymes at last to the digestion mixture.

Introduction: The endonucleases are the enzymes, which are also called restriction enzymes or restriction endonucleases that are able to cleave the phosphodiester bonds of the DNA. These restriction endonucleases are able to cut the DNA at any specific ends. The use of restriction endonucleases has specific importance in the field of recombinant DNA technology.

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