Microbiology: A Laboratory Manual (11th Edition)
11th Edition
ISBN: 9780134098630
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 14, Problem 57E.7RQ
a.
Summary Introduction
To list: The different forms of a plasmid isolated from the bacterial cells.
Introduction: The plasmids are small, circular extrachromosomal DNA that exists separately from the genome of the host cell. Plasmids can self-replicate and are also used as cloning vectors. The plasmids that are used as cloning vectors consists of an origin of replication, a cloning site, and a selectable marker.
b.
Summary Introduction
To explain: The plasmid form that migrates fastest and farthest in an electrophoresis experiment.
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8. Transcription and Translation Practice: (Video 10-1 and 10-2)
A. Below is the sense strand of a DNA gene. Using the sense strand, create the antisense
DNA strand and label the 5' and 3' ends.
B. Use the antisense strand that you create in part A as a template to create the mRNA
transcript of the gene and label the 5' and 3' ends.
C. Translate the mRNA you produced in part B into the polypeptide sequence making sure
to follow all the rules of translation.
5'-AGCATGACTAATAGTTGTTGAGCTGTC-3' (sense strand)
4
What is the structure and function of Eukaryotic cells, including their organelles? How are Eukaryotic cells different than Prokaryotic cells, in terms of evolution which form of the cell might have came first? How do Eukaryotic cells become malignant (cancerous)?
What are the roles of DNA and proteins inside of the cell? What are the building blocks or molecular components of the DNA and proteins? How are proteins produced within the cell? What connection is there between DNA, proteins, and the cell cycle? What is the relationship between DNA, proteins, and Cancer?
Chapter 14 Solutions
Microbiology: A Laboratory Manual (11th Edition)
Ch. 14 - Prob. 56E.1RQCh. 14 - Prob. 56E.2RQCh. 14 - Prob. 56E.3RQCh. 14 - Why is plasmid DNA preferred for genetic...Ch. 14 - What are selectable markers, and why are they...Ch. 14 - Prob. 57E.3RQCh. 14 - Alcohol is considered to be a significant reagent...Ch. 14 - Prob. 57E.5RQCh. 14 - Prob. 57E.6RQCh. 14 - Prob. 57E.7RQ
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