(a) Interpretation: The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined. Concept introduction: The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Answer 1

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)

a) What are the differences between the Direct & Indirect Immunofluorescence Assays? b) What are the advantages of the Indirect Immunofluorescence Assays? c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?

Answer 2

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Expert Solution & Answer

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Answer 3

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

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Answer 4

Question

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Expert Solution & Answer

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Answer 5

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 6

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 7

Chapter 14, Problem 22P

Interpretation Introduction

(a)

Interpretation:

The reason for the total binding not to be an accurate representation of the total number of cell-surface receptors should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The purpose of performing the experiment in the presence of excess nonradioactive ligand must be determined.

Concept introduction:

The ligand binding assay is an analytic procedure, in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The significance of specific binding attaining a plateau should be determined.

Concept introduction:

The ligand binding assay is an analytic procedure in which the ligand-receptor binding is the basis of the experiment. By the fluorescence detection method or electrochemical ways, the presence and affinity (extent) of ligand-receptor binding can be determined.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 14 - Prob. 1P Ch. 14 - Prob. 2P Ch. 14 - Prob. 3P Ch. 14 - Prob. 4P Ch. 14 - Prob. 5P Ch. 14 - Prob. 6P Ch. 14 - Prob. 7P Ch. 14 - Prob. 8P Ch. 14 - Prob. 9P Ch. 14 - Prob. 10P

Solution Summary: The author explains that the ligand binding assay is an analytic procedure, in which the receptor binding is the basis of the experiment.

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Chapter 14 Solutions