Genetics: From Genes to Genomes
6th Edition
ISBN: 9781259700903
Author: Leland Hartwell Dr., Michael L. Goldberg Professor Dr., Janice Fischer, Leroy Hood Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 14, Problem 15P
E. coli cells usually have only one copy of the F plasmid per cell. You have isolated a cell in which a mutation increases the copy number of F to three to four per cell. How could you distinguish between the possibility that the copy number change was due to a mutation in the F plasmid versus a mutation in a chromosomal gene?
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You transform bacteria with a plasmid carrying the ampicillin-resistance gene ampR. How would you determine which bacteria took up the
plasmid?
O Bacteria containing the plasmid would be able to grow in the absence of ampicillin.
O Bacteria containing the plasmid would be able to grow in the presence of ampicillin.
O Bacteria containing the plasmid would be able to produce ampicillin.
O Ampicillin-resistance is necessary for the plasmid DNA
get into the bacteria.
You have two E. coli strains, XL10 and K12.
XL10 is ampicillin resistant, or AmpR, and can grow on lab media plates in the presence of
ampicillin.
K12 is ampicillin sensitive, or AmpS, and cannot grow on lab media plates in the presence
of ampicillin.
You suspect that the AmpR gene is carried on a plasmid in XL10, rather than on its
chromosome, and want to design an experiment to test this hypothesis.
In designing your experiment, you may assume that you have access to the
following materials:
all reagents, plates, plasmids, and equipment used in the lab this semester
Overnight cultures of XL10 & K12
Competent cells of XL10 & K12
a. Describe briefly the steps of a simple experiment that you could perform to test
the hypothesis that the AmpR gene is carried on a plasmid in XL10 and not on its
chromosome. (NOTE: no need for detailed procedure, simply state what you would
do is sufficient).
b. What specific experimental result will tell you that your hypothesis is correct?
In several experiments, you find these orders of genes getting transferred in from the chromosome with
integrated F plasmid. Note the experimental results are listed vertically this time.
Expt3
K
Exptl
Expt2
Expt4
Expt5
A
J
E
D
F
N
B
M
L
H
F
P
A
I
I
H
C
J
H
L
Use this data to create a map. .
draw the correct "shape" of a map.
Chapter 14 Solutions
Genetics: From Genes to Genomes
Ch. 14 - Choose the phrase from the right column that best...Ch. 14 - The unicellular, rod-shaped bacterium E. coli is 2...Ch. 14 - Now that the sequence of the entire E. coli K12...Ch. 14 - Bacterial genomes such as that of E. coli...Ch. 14 - List at least three features of eukaryotic genomes...Ch. 14 - Describe a mechanism by which a gene could move...Ch. 14 - High salt concentrations tend to cause protein...Ch. 14 - Recently, scientists tested the possibility that...Ch. 14 - A recent metagenomic study analyzed the...Ch. 14 - Linezolid is a new type of antibiotic that...
Ch. 14 - A liquid culture of E. coli at a concentration of...Ch. 14 - Pick out the medium i, ii, iii, or iv onto which...Ch. 14 - This problem concerns Fig. 14.14, which...Ch. 14 - In two isolates one is resistant to ampicillin,...Ch. 14 - E. coli cells usually have only one copy of the F...Ch. 14 - In E. coli, the genes purC and pyrB are located...Ch. 14 - DNA sequencing of the entire H. influenzae genome...Ch. 14 - Genes encoding toxins are often located on...Ch. 14 - a. You want to perform an interrupted-mating...Ch. 14 - In Problem 19, do you think that most of the...Ch. 14 - One issue with interrupted-mating experiments such...Ch. 14 - Prob. 22PCh. 14 - Starting with an F- strain that was prototrophic...Ch. 14 - You can carry out matings between an Hfr and F...Ch. 14 - Genome sequences show that some pathogenic...Ch. 14 - Generalized and specialized transduction both...Ch. 14 - This problem highlights some useful variations of...Ch. 14 - A researcher has a Trp auxotrophic strain of E....Ch. 14 - Streptococcus parasanguis is a bacterial species...Ch. 14 - The sequence at one end of one strand of the...Ch. 14 - Scientists who study amino acid biosynthesis...Ch. 14 - Suppose that you could obtain radioactively...Ch. 14 - Prob. 34PCh. 14 - Some scientists are trying to engineer...
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- In Figure 5-15, how are each of the following genotypesproduced?a. F+ a− c. F− a+b. F− a− d. F+ a+arrow_forwardAfter transformation you were asked to grow bacterial cells transformed with plasmid on a plate that had X-gal and ampicillin. X-Gal is often used as in indicator dye, which turns blue when metabolized by B-galactosidase protein and used to test if cloning experiments have worked. [Note look at the vector diagrams carefully] Briefly explain how you would find the bacterial cells that are transformed with the plasmid with the YFG inserted.arrow_forwardExplain why the genes whose products are required for the normal growth of bacteria not carried on plasmids? Give two examples (one bacterial gene and one plasmid gene) to support your answer.arrow_forward
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