BIOLOGY
12th Edition
ISBN: 9781264839698
Author: Raven
Publisher: MCG CUSTOM
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Chapter 13.3, Problem 1LO
Summary Introduction
To explain: The inheritance pattern for genes compacted in the DNA of mitochondrion and chloroplast.
Introduction: All eukaryotic organisms contain chromosomes, and the number of chromosomes varies in different organisms. In humans and several other species, diploid (2n) number of chromosomes is present. The diploid number of chromosomes indicates that each parent contributes equally to the offspring. Therefore for humans, the diploid and haploid number is 46 and 23, respectively. Of these, 22 pairs are perfectly similar in both females and males and are referred to as autosomes. The rest of the pair is referred to as sex chromosomes: XY in males and XX in females. Doubling of gene products (sex-linked) is ultimately prevented by dosage compensation
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 13 Solutions
BIOLOGY
Ch. 13.1 - Describe sex-linked inheritance in fruit flies.Ch. 13.1 - Prob. 2LOCh. 13.2 - Prob. 1LOCh. 13.2 - Explain the genetic consequences of dosage...Ch. 13.3 - Prob. 1LOCh. 13.4 - Prob. 1LOCh. 13.4 - Explain the relationship between frequency of...Ch. 13.4 - Prob. 3LOCh. 13.5 - Prob. 1LOCh. 13.5 - Prob. 2LO
Ch. 13.5 - Prob. 3LOCh. 13 - Inquiry question Mendel did not examine plant...Ch. 13 - What would Mendel have observed in a dihybrid...Ch. 13 - Prob. 2DACh. 13 - Why is the white-eye phenotype always observed in...Ch. 13 - In an organisms genome, autosomes are a. the...Ch. 13 - What cellular process is responsible for genetic...Ch. 13 - The map distance between two genes is determined...Ch. 13 - How many map units separate two alleles if the...Ch. 13 - How does maternal inheritance of mitochondrial...Ch. 13 - Which of the following genotypes due to...Ch. 13 - Prob. 1ACh. 13 - As real genetic distance increases, the distance...Ch. 13 - Down syndrome is the result of trisomy for...Ch. 13 - Genes that are on the same chromosome can show...Ch. 13 - The A and B genes are 10 cM apart on a chromosome....Ch. 13 - Prob. 6ACh. 13 - Color blindness is caused by a sex-linked,...Ch. 13 - Assume that the genes for seed color and seed...Ch. 13 - A low frequency of calico cats are male (about...
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