BIOLOGY
12th Edition
ISBN: 9781264839698
Author: Raven
Publisher: MCG CUSTOM
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Chapter 13, Problem 2DA
Summary Introduction
To determine: The reason that the chances of Down syndrome increase with maternal age.
Introduction: Down syndrome is the disease that is caused due to the presence of an extra copy of chromosome 21 or trisomy of the 21st chromosome. It occurs due to non-disjunction of the autosomal chromosome.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 13 Solutions
BIOLOGY
Ch. 13.1 - Describe sex-linked inheritance in fruit flies.Ch. 13.1 - Prob. 2LOCh. 13.2 - Prob. 1LOCh. 13.2 - Explain the genetic consequences of dosage...Ch. 13.3 - Prob. 1LOCh. 13.4 - Prob. 1LOCh. 13.4 - Explain the relationship between frequency of...Ch. 13.4 - Prob. 3LOCh. 13.5 - Prob. 1LOCh. 13.5 - Prob. 2LO
Ch. 13.5 - Prob. 3LOCh. 13 - Inquiry question Mendel did not examine plant...Ch. 13 - What would Mendel have observed in a dihybrid...Ch. 13 - Prob. 2DACh. 13 - Why is the white-eye phenotype always observed in...Ch. 13 - In an organisms genome, autosomes are a. the...Ch. 13 - What cellular process is responsible for genetic...Ch. 13 - The map distance between two genes is determined...Ch. 13 - How many map units separate two alleles if the...Ch. 13 - How does maternal inheritance of mitochondrial...Ch. 13 - Which of the following genotypes due to...Ch. 13 - Prob. 1ACh. 13 - As real genetic distance increases, the distance...Ch. 13 - Down syndrome is the result of trisomy for...Ch. 13 - Genes that are on the same chromosome can show...Ch. 13 - The A and B genes are 10 cM apart on a chromosome....Ch. 13 - Prob. 6ACh. 13 - Color blindness is caused by a sex-linked,...Ch. 13 - Assume that the genes for seed color and seed...Ch. 13 - A low frequency of calico cats are male (about...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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- is often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forward
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