CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
8th Edition
ISBN: 9781259880193
Author: SALADIN
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 13.2, Problem 6BYGO
Summary Introduction
Introduction:
The spinal nerves are important because the spinal cord sends and receives signals from the body through the spinal nerves. The spinal nerve is composed of large number of nerve fibers or axons and resembles a cordlike appearance. The nervous tissue of the spinal cord is composed of organized cells that involve in the body movements and send the signals to different parts of the body. The spinal cord tracts are stimulatory nerve pathways that pass the information through action potentials.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 13 Solutions
CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
Ch. 13.1 - Prob. 1AYLOCh. 13.1 - Prob. 2AYLOCh. 13.1 - Prob. 3AYLOCh. 13.1 - Prob. 4AYLOCh. 13.1 - Prob. 5AYLOCh. 13.1 - Prob. 6AYLOCh. 13.1 - Prob. 7AYLOCh. 13.1 - Prob. 8AYLOCh. 13.1 - The position of the posterior and anterior horns...Ch. 13.1 - Prob. 10AYLO
Ch. 13.1 - Prob. 11AYLOCh. 13.1 - Prob. 12AYLOCh. 13.1 - Names and functions of the descending tractsCh. 13.1 - Prob. 14AYLOCh. 13.1 - Prob. 15AYLOCh. 13.1 - Prob. 16AYLOCh. 13.1 - Prob. 1BYGOCh. 13.1 - Prob. 2BYGOCh. 13.1 - Prob. 3BYGOCh. 13.1 - Prob. 4BYGOCh. 13.1 - Prob. 5BYGOCh. 13.2 - Prob. 1AYLOCh. 13.2 - Prob. 2AYLOCh. 13.2 - Prob. 3AYLOCh. 13.2 - Prob. 4AYLOCh. 13.2 - Prob. 5AYLOCh. 13.2 - Prob. 6AYLOCh. 13.2 - Prob. 7AYLOCh. 13.2 - Prob. 8AYLOCh. 13.2 - Prob. 9AYLOCh. 13.2 - Prob. 10AYLOCh. 13.2 - Prob. 11AYLOCh. 13.2 - Prob. 12AYLOCh. 13.2 - Prob. 6BYGOCh. 13.2 - Prob. 7BYGOCh. 13.2 - Prob. 8BYGOCh. 13.2 - Prob. 9BYGOCh. 13.3 - Prob. 1AYLOCh. 13.3 - The pathway and constituents of a somatic reflex...Ch. 13.3 - Prob. 3AYLOCh. 13.3 - Structure and function of muscle spindlesCh. 13.3 - Stretch reflexes; one or more examples; the...Ch. 13.3 - Prob. 6AYLOCh. 13.3 - Prob. 7AYLOCh. 13.3 - Prob. 8AYLOCh. 13.3 - Prob. 9AYLOCh. 13.3 - Name five structural components of a typical...Ch. 13.3 - State the function of each of the following in a...Ch. 13.3 - Prob. 12BYGOCh. 13.3 - Prob. 13BYGOCh. 13.3 - Prob. 14BYGOCh. 13 - arachno-Ch. 13 - Prob. 2BYMVCh. 13 - Prob. 3BYMVCh. 13 - Prob. 4BYMVCh. 13 - Prob. 5BYMVCh. 13 - Prob. 6BYMVCh. 13 - Prob. 7BYMVCh. 13 - Prob. 8BYMVCh. 13 - Prob. 9BYMVCh. 13 - tecto-Ch. 13 - Below L2. the vertebral canal is occupied by a...Ch. 13 - Prob. 2TYRCh. 13 - Nerve fibers that adjust the tension in a muscle...Ch. 13 - A stretch reflex requires the action of _______ to...Ch. 13 - Prob. 5TYRCh. 13 - Prob. 6TYRCh. 13 - Prob. 7TYRCh. 13 - Prob. 8TYRCh. 13 - Prob. 9TYRCh. 13 - Prob. 10TYRCh. 13 - Prob. 11TYRCh. 13 - Prob. 12TYRCh. 13 - The cerebellum receives feedback from the muscles...Ch. 13 - In the ______ reflex, contraction of flexor...Ch. 13 - Prob. 15TYRCh. 13 - Prob. 16TYRCh. 13 - Prob. 17TYRCh. 13 - Prob. 18TYRCh. 13 - Prob. 19TYRCh. 13 - Prob. 20TYRCh. 13 - Prob. 1WWTSCh. 13 - Prob. 2WWTSCh. 13 - Prob. 3WWTSCh. 13 - Some spinal nerves are sensory and others are...Ch. 13 - Prob. 5WWTSCh. 13 - The anterior and posterior horns of the spinal...Ch. 13 - Prob. 7WWTSCh. 13 - Prob. 8WWTSCh. 13 - Prob. 9WWTSCh. 13 - Prob. 10WWTSCh. 13 - Jillian is thrown from a horse. She strikes the...Ch. 13 - Wallace is the victim of a hunting accident. A...Ch. 13 - Anthony gets into a fight between rival gangs As...Ch. 13 - Stand with your right shoulder, hip, and foot...Ch. 13 - When a patient needs a tendon graft, surgeons...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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