Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780321962751
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Textbook Question
Chapter 13, Problem 3TYU
In analyzing the number of different bases in a DNA sample, which result would be consistent with the base-pairing rules?
- A. A=G
- B. A + G = C + T
- C. A+ T = G + C
- D. A=C
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What is the purpose of Bromophenol Blue in electrophoresis?
a.
To follow the progress of the gel as it is running
b.
It acts as a DNA size ladder or DNA size standards.
c.
To allow the electric current to move through the gel.
d.
To visualize the DNA after the gel has finished running.
What is the purpose of acetate in TAE?
a.
It helps to maintain the pH of the buffer
b.
To carry electrons to allow the electric current to travel through the gel.
c.
It is used to stop the action of DNases
d.
It is required to see the DNA on a UV light.
What feature of a DNA fragment causes it to move through a gel during electrophoresis? a. the electrical charges of its phosphate groups b. its nucleotide sequence c. the hydrogen bonds between its base pairs d. its double helix shape
Which part of the DNA is broken in a double strand break?
a. Hydrogen bonds between bases
b. Covalent bonds between the base and the sugar
c. Covalent bonds between the base and the 2'-OH group
d. Covalent bonds between the 3'OH of one nucleotide and the 5' phosphate of the next nucleotide
Chapter 13 Solutions
Campbell Biology in Focus (2nd Edition)
Ch. 13.1 - Given a polynucleotide sequence such as GAATTC,...Ch. 13.1 - Prob. 2CCCh. 13.2 - What role does base pairing play in the...Ch. 13.2 - Make a table listing the functions of seven...Ch. 13.2 - MAKE CONNECTIONS What is the relationship between...Ch. 13.3 - Describe the structure of a nucleosome, the basic...Ch. 13.3 - What two properties, one structural and one...Ch. 13.4 - Prob. 1CCCh. 13.4 - DRAW IT One strand of a DNA molecule has the...Ch. 13.4 - Describe the role of complementary base pairing...
Ch. 13 - In his work with pneumonia-causing bacteria and...Ch. 13 - What is the basis for the difference in how the...Ch. 13 - In analyzing the number of different bases in a...Ch. 13 - The elongation of the leading strand during DNA...Ch. 13 - Prob. 5TYUCh. 13 - Prob. 6TYUCh. 13 - Prob. 7TYUCh. 13 - Prob. 8TYUCh. 13 - Prob. 9TYUCh. 13 - MAKE CONNECTIONS Although the proteins that cause...Ch. 13 - Prob. 12TYUCh. 13 - FOCUS ON EVOLUTION Some bacteria may be able to...Ch. 13 - FOCUS ON ORGANIZATION The continuity of life is...
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- When working with the gel electrophoresis chamber, what must we keep in mind? A. Keeping the gel flat on the table without any buffer solution B. Not changing your pipette in between DNA samples C. Not shaking the chamber after the DNA has been loaded D. Loading two DNA samples per a single wellarrow_forwardHow do amino acids in DNA-binding proteins interact with DNA? a. By forming covalent bonds with DNA bases b. By forming hydrogen bonds with DNA bases c. By forming covalent bonds with DNA sugarsarrow_forwardWhich is a difference between B-DNA and A-DNA? A. For polynucleotide strands containing the same number of nucleotides, the B-DNA strand will be shorter from end-to-end than the corresponding A-DNA. B. Both are helical, but B-DNA is right-handed and A-DNA is left-handed. C. The sugar in A-DNA is more oxidized than that in B-DNA D. Helical structures in RNA predominantly adopt the B-DNA conformation. E. None of the above. The tetranucleotide AGTC (in DNA) has a free hydroxyl group on ____. A. A B. G, T, and C C. C D. A, G, T, and Carrow_forward
- Choose the combination of answers that most accurately completes the statement. Why must the lagging strand of DNA be replicated in short pieces? a. because of limited space b. otherwise, the helix will become distorted c. the DNA polymerase can synthesize in only one direction d. to make proofreading of code easierarrow_forwardChoose the combination of answers that most accurately completes the statement.Why must the lagging strand of DNA be replicated in short pieces? a. because of limited space b. otherwise, the helix will become distorted c. the DNA polymerase can synthesize in only one direction d. to make proofreading of code easierarrow_forwardIf the DNA of an organism contains 20% adenine, what is its percentage of guanine? a.20% b.60% c.80% d.30%arrow_forward
- What is the role of Sodium Iodidee in DNA isolation? A. Solubilization of phospholipids B Precipitation of DNA C Destroy hydrogen bonding network in solvent D Precipitation of cel wall and cell membranearrow_forwardWhy do the fragments of DNA in gel electrophoresis travel away from the negative electrode? A. DNA is positively charged to attracted to the negative end of the unit B. the agarose gel is positively charged C. the agarose gel in negatively charged D. DNA is negatively charged so attracted to the positive end of the unitarrow_forwardWhich is the 3' end of this DNA stand? A. B. С. O The location marked with the red A. O The location marked with the red B. The location marked with the red C.arrow_forward
- What is the role of Sodum Dodecyl Sulffate in DNA isolation? A. Precipitate DNA B Make the solution more viscous C Destroy the cell membrane D Maintain ionic strengtharrow_forwardWhen two adjacent bases in the same strand of DNA dimerize (form a covalent bond between them), what happens to the DNA? a. the original strand of DNA now contains a new DNA sequence b. the original strand of DNA is prevented from opening during replication, so this section of DNA will not be replicated c. the original strand of DNA is methylated, which causes the bases to mismatch d. the original strand of DNA is kinked, which prevents DNA polymerase from working properly e. the original strand of DNA is unaffected, so no additional mutations arisearrow_forwardYou are studying bacterial cells and you find bacteria with a mutation that causes it to produce non-functional ligase. If you allowed that cell to replicate once and then you isolated the DNA from the two newly formed cells what would you expect to find? A. One longer single strand of DNA and many short single strands of DNA B. Two longer single strands of DNA and many short single strands of DNA C. Four longer single strands of DNA and many short single strands of DNA D. Eight longer single strands of DNA and many short single strands of DNA Choose the correct answer above and explain why you chose that answer. Your explanation should discuss the replication of DNA in bacteria and the role of ligase in DNA replication.arrow_forward
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