Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781260163698
Author: Cowan
Publisher: MCG
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Chapter 12.1, Problem 8AYP
Summary Introduction
To determine:
The importance of cytokines and to give one example of pro-inflammatory and anti-inflammatory cytokine.
Concept introduction:
Cytokines are number of substances, such as interferon and growth factors, which are secreted by white blood cells of the immune system and they have an effect on other cells.
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a) The lux operon is under positive control. Based on this information, does the luxR regulator sequence make a repressor protein or an activator protein?
b) How will binding of this complex affect RNA polymerase? Remember this operon is under positive control.
c) AHL is a signal molecule that V. fisheri makes to communicate with neighboring bacterial cells. This molecule can diffuse outside of the cell and into another bacterial cell in close proximity. This type of communication between bacterial cells is known as quorum sensing. If bacterial cell density is low how will this affect the lux operon? What will happen if the density is high?
1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’
“met-_________-_________-_________-_________-_________”STOP”
2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results.
Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn
A. Missense
B. Silent
C. Nonsense
D. Frameshift
______ Pro-Thr-Ser-STOP
______ Pro-Thr-Ser-Leu-Ile-His-Asn
______ Pro-Thr-Ser-Leu-Leu-His-Asn
_______ Pro-Thr-His-Cys-Tyr-Thr
Chapter 12 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 12.1 - Summarize the three lines of host defenses.Ch. 12.1 - Define marker and discuss its importance in the...Ch. 12.1 - Prob. 3AYPCh. 12.1 - Prob. 4AYPCh. 12.1 - Name three kinds of blood cells that function in...Ch. 12.1 - Connect the mononuclear phagocyte system to innate...Ch. 12.1 - Describe how T and B lymphocytes are involved in...Ch. 12.1 - Prob. 8AYPCh. 12.1 - Prob. 1MMCh. 12.1 - Prob. 1NP
Ch. 12.1 - Prob. 2MMCh. 12.2 - Prob. 9AYPCh. 12.2 - Prob. 10AYPCh. 12.2 - Prob. 11AYPCh. 12.2 - Prob. 2NPCh. 12.3 - List the four major categories of nonspecific...Ch. 12.3 - Prob. 13AYPCh. 12.3 - Prob. 14AYPCh. 12.3 - Prob. 15AYPCh. 12.3 - Name four types of antimicrobial host-derived...Ch. 12.3 - Prob. 17AYPCh. 12.3 - Prob. 3NPCh. 12.3 - NCLEX PREX 4. A patient presents to the emergency...Ch. 12.3 - Prob. 5NPCh. 12 - A microorganism carries _____ markers and a B cell...Ch. 12 - Prob. 2QCh. 12 - The pediatrician you work for has just recommended...Ch. 12 - Prob. 4QCh. 12 - In what way is a phagocyte a tiny container of...Ch. 12 - Prob. 6QCh. 12 - Prob. 7QCh. 12 - Prob. 8QCh. 12 - Explain the physiological events that lead to each...Ch. 12 - Prob. 10QCh. 12 - Prob. 11QCh. 12 - Prob. 12QCh. 12 - Prob. 13QCh. 12 - Why do you think that the intestines have one of...Ch. 12 - Prob. 15QCh. 12 - Prob. 16QCh. 12 - Prob. 17QCh. 12 - Prob. 18QCh. 12 - Figure 12.3 demonstrates that lymphatic fluid...Ch. 12 - Prob. 20QCh. 12 - Prob. 21QCh. 12 - Prob. 1VC
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- a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________arrow_forward1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forward
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