Microbiology Fundamentals: A Clinical Approach
3rd Edition
ISBN: 9781260163698
Author: Cowan
Publisher: MCG
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Chapter 12.3, Problem 3NP
Summary Introduction
Introduction:
White blood cells play important role in the body’s immune system. They protect the body against any infections and pathogens. There are five types of WBC; neutrophils, lymphocytes, monocytes, eosinophils, and basophils.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 12 Solutions
Microbiology Fundamentals: A Clinical Approach
Ch. 12.1 - Summarize the three lines of host defenses.Ch. 12.1 - Define marker and discuss its importance in the...Ch. 12.1 - Prob. 3AYPCh. 12.1 - Prob. 4AYPCh. 12.1 - Name three kinds of blood cells that function in...Ch. 12.1 - Connect the mononuclear phagocyte system to innate...Ch. 12.1 - Describe how T and B lymphocytes are involved in...Ch. 12.1 - Prob. 8AYPCh. 12.1 - Prob. 1MMCh. 12.1 - Prob. 1NP
Ch. 12.1 - Prob. 2MMCh. 12.2 - Prob. 9AYPCh. 12.2 - Prob. 10AYPCh. 12.2 - Prob. 11AYPCh. 12.2 - Prob. 2NPCh. 12.3 - List the four major categories of nonspecific...Ch. 12.3 - Prob. 13AYPCh. 12.3 - Prob. 14AYPCh. 12.3 - Prob. 15AYPCh. 12.3 - Name four types of antimicrobial host-derived...Ch. 12.3 - Prob. 17AYPCh. 12.3 - Prob. 3NPCh. 12.3 - NCLEX PREX 4. A patient presents to the emergency...Ch. 12.3 - Prob. 5NPCh. 12 - A microorganism carries _____ markers and a B cell...Ch. 12 - Prob. 2QCh. 12 - The pediatrician you work for has just recommended...Ch. 12 - Prob. 4QCh. 12 - In what way is a phagocyte a tiny container of...Ch. 12 - Prob. 6QCh. 12 - Prob. 7QCh. 12 - Prob. 8QCh. 12 - Explain the physiological events that lead to each...Ch. 12 - Prob. 10QCh. 12 - Prob. 11QCh. 12 - Prob. 12QCh. 12 - Prob. 13QCh. 12 - Why do you think that the intestines have one of...Ch. 12 - Prob. 15QCh. 12 - Prob. 16QCh. 12 - Prob. 17QCh. 12 - Prob. 18QCh. 12 - Figure 12.3 demonstrates that lymphatic fluid...Ch. 12 - Prob. 20QCh. 12 - Prob. 21QCh. 12 - Prob. 1VC
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
- Which of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forwardWhat is the "gold standard" that is currently applied to species designations in microbiology? 97% between species: 50% among whole genome 90% between species: 75% among whole genome 99% between species; 97% among whole genome 97% between species: 70% among whole genome Onone of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have decided to send your sample off for sequencing. You need to determine which type of sequencing to use for the preliminary identification of your species. You decide that, for now, you only need to be able to identify the family and genus levels. Which type of sequencing do you think is the most appropriate? O Genomic Fingerprinting O Whole Genome Sequencing O 16S rDNA Sequencing O DNA-DNA hybridization Nextarrow_forward
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