Human Anatomy
5th Edition
ISBN: 9780073403700
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 12, Problem 9TYR
Both the gastrocnemius and ____________ muscles insert on the heel by way of the calcaneal insert on the heel by way of the calcaneal tendon.
- semimembranosus
- tibialis posterior
- tibialis anterior
- soleus
- plantaris
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 12 Solutions
Human Anatomy
Ch. 12.2 - Prob. 1AWYKCh. 12.2 - Prob. 2AWYKCh. 12.2 - Prob. 1BYGOCh. 12.2 - Prob. 2BYGOCh. 12.2 - Prob. 3BYGOCh. 12.2 - Prob. 4BYGOCh. 12.2 - Prob. 5BYGOCh. 12.2 - Prob. 6BYGOCh. 12.2 - Not everyone has the same muscles. From the...Ch. 12.2 - Prob. 7BYGO
Ch. 12.2 - Name the muscles that cross both the hip and knee...Ch. 12.2 - Prob. 9BYGOCh. 12.2 - Prob. 10BYGOCh. 12.2 - Prob. 11BYGOCh. 12.2 - Prob. 12BYGOCh. 12 - Prob. 12.1.1AYLOCh. 12 - Prob. 12.1.2AYLOCh. 12 - Prob. 12.1.3AYLOCh. 12 - Prob. 12.1.4AYLOCh. 12 - Prob. 12.1.5AYLOCh. 12 - Prob. 12.1.6AYLOCh. 12 - Prob. 12.1.7AYLOCh. 12 - Prob. 12.1.8AYLOCh. 12 - Prob. 12.1.9AYLOCh. 12 - Prob. 12.1.10AYLOCh. 12 - Prob. 12.1.11AYLOCh. 12 - Prob. 12.1.12AYLOCh. 12 - Prob. 12.1.13AYLOCh. 12 - Prob. 12.1.14AYLOCh. 12 - Prob. 12.1.15AYLOCh. 12 - Prob. 12.1.16AYLOCh. 12 - Prob. 12.2.1AYLOCh. 12 - The three gluteal muscles-gluteus maximus, gluteus...Ch. 12 - The tensor fasciae latae of the hip and its...Ch. 12 - Prob. 12.2.4AYLOCh. 12 - Prob. 12.2.5AYLOCh. 12 - Prob. 12.2.6AYLOCh. 12 - Prob. 12.2.7AYLOCh. 12 - The hamstring muscles of the posterior compartment...Ch. 12 - Prob. 12.2.9AYLOCh. 12 - Prob. 12.2.10AYLOCh. 12 - Prob. 12.2.11AYLOCh. 12 - Prob. 12.2.12AYLOCh. 12 - Prob. 12.2.13AYLOCh. 12 - Prob. 12.2.14AYLOCh. 12 - Prob. 12.2.15AYLOCh. 12 - Prob. 12.3.1AYLOCh. 12 - Prob. 12.3.2AYLOCh. 12 - Prob. 1TYRCh. 12 - Prob. 2TYRCh. 12 - Prob. 3TYRCh. 12 - Prob. 4TYRCh. 12 - Which of these muscles does not contribute to the...Ch. 12 - Which of these actions is not performed by the...Ch. 12 - Prob. 7TYRCh. 12 - Prob. 8TYRCh. 12 - Both the gastrocnemius and ____________ muscles...Ch. 12 - Prob. 10TYRCh. 12 - Prob. 11TYRCh. 12 - Prob. 12TYRCh. 12 - Pronation of the forearm is achieved by two...Ch. 12 - Prob. 14TYRCh. 12 - Prob. 15TYRCh. 12 - Prob. 16TYRCh. 12 - Prob. 17TYRCh. 12 - Prob. 18TYRCh. 12 - Prob. 19TYRCh. 12 - Prob. 20TYRCh. 12 - Prob. 1BYMVCh. 12 - Prob. 2BYMVCh. 12 - State a meaning of each world element and give a...Ch. 12 - Prob. 4BYMVCh. 12 - Prob. 5BYMVCh. 12 - Prob. 6BYMVCh. 12 - State a meaning of each world element and give a...Ch. 12 - Prob. 8BYMVCh. 12 - Prob. 9BYMVCh. 12 - Prob. 10BYMVCh. 12 - Briefly explain why each of the following...Ch. 12 - Determine which five of the following statements...Ch. 12 - Prob. 3TOFCh. 12 - Determine which five of the following statements...Ch. 12 - Prob. 5TOFCh. 12 - Prob. 6TOFCh. 12 - Briefly explain why each of the following...Ch. 12 - Prob. 8TOFCh. 12 - Prob. 9TOFCh. 12 - Prob. 10TOFCh. 12 - Prob. 1TYCCh. 12 - Prob. 2TYCCh. 12 - Poorly conditioned middle-aged people may suffer a...Ch. 12 - Women who habitually wear high heels may suffer...Ch. 12 - A student moving out of a dormitory crouches in...
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