Chapter 12, Problem 9CTQ

a.

Summary Introduction

To explain:

The reason as to why growth on the plate was observed after incubation of a sample aseptically obtained from growth free portion of the zone of inhibition at $37\text{°C}$ for 48 hours. Also, to determine how this result impacts analysis of the Kirby-Bauer test.

Concept introduction:

Kirby-Bauer test is also called “disc diffusion antibiotic sensitivity test”. It is a test to determine the antibiotic sensitivity of bacteria. In this test, antibiotic discs are placed in Petri plates containing bacterial culture. A particular zone of inhibition around the disc represents the bacterium in the culture is susceptible to that antibiotic.

b.

Summary Introduction

To determine:

Whether amoxicillin (the tested drug) is an effective drug choice for the treatment of infection caused by the cultured bacterium.

Concept introduction:

Kirby-Bauer test is also called “disc diffusion antibiotic sensitivity test”. It is a test to determine the antibiotic sensitivity of bacteria. In this test, antibiotic discs are placed in Petri plates containing bacterial culture. A particular zone of inhibition around the disc represents the bacterium in the culture is susceptible to that antibiotic.