Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 12, Problem 3RQ
Summary Introduction
To review:
The important characteristics of reporter genes used for the measurement of transcriptional and translational control.
Introduction:
Reporter genes are used to monitor the expression of specific genes. They are attached to the regulatory sequence of another gene in order to study the function of these sequences. These genes express easily observable traits that eases the identification of cells expressing them.
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Chapter 12 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 12.1 - Prob. 1TQCh. 12.1 - Prob. 2TQCh. 12.1 - Prob. 3TQCh. 12.1 - Prob. 4TQCh. 12.2 - Prob. 1TQCh. 12.2 - Prob. 2TQCh. 12.2 - Prob. 3TQCh. 12.3 - Prob. 1TQCh. 12.4 - Prob. 1TQCh. 12.4 - Prob. 2TQ
Ch. 12.5 - Prob. 1TQCh. 12 - Prob. 1RQCh. 12 - Prob. 2RQCh. 12 - Prob. 3RQCh. 12 - Prob. 4RQCh. 12 - Prob. 5RQCh. 12 - Prob. 6RQCh. 12 - Prob. 7RQCh. 12 - Prob. 8RQCh. 12 - Prob. 9RQCh. 12 - Prob. 10RQCh. 12 - Prob. 11RQCh. 12 - Prob. 12RQCh. 12 - Prob. 13RQCh. 12 - Prob. 14RQCh. 12 - Prob. 15RQCh. 12 - Prob. 1TQCh. 12 - Prob. 2TQCh. 12 - Prob. 3TQ
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- What enzyme catalyzes transcription? What is a promoter and what protein recognizes promoters in Bacteria?arrow_forwardWhat is the relationship between promoters, regulatory DNA sequences, and transcription regulators? How are they used to control gene expression?arrow_forwardWhich of the following methods is utilized by eukaryotes to control their gene expression that is different from the type of control found in bacteria? a) control of chromatic remodeling. b) control of RNA splicing. c) transcriptional control. d) control of both RNA splicing and chromatin remodeling.arrow_forward
- What are the transcription factors in eukaryotes? What is the role of transcription factors? What are the two types of transcription factors?arrow_forwardWhat are the functions of transcriptional activator proteins and repressor proteins? Explain how they work at the molecular level.arrow_forwardWhat are the cytoplasmic mechanisms of Post Transcriptional control?arrow_forward
- 2) what is negative control of genes ? Does it need active form or inactive form of the repressor ? 3) What is role of RNA polymerase, operator and the repressor in repressible operon and inducible operons ? 4) How is eukaryotic gene expression different than bacterial gene expression ? 5) How does histone acetylation influence transcription ?arrow_forwardWhat are the similarities and differences in the initiation of gene transcription in bacteria and eukaryotes?arrow_forwardi)Describe attenuation control and how it is used to regulate gene expression. ii)Give a specific example of how this works? iii)Could this be used in eukaryotes? why ?or why not?arrow_forward
- A) List the steps for gene expression in prokaryotes and eukaryotes. B) Relate the differences in gene expression between prokaryotes and eukaryotes in gene expression regulation and explain what causes those differences.arrow_forwardCompare the control of gene regulation in eukaryotes and bacteria at the level of initiation of transcription. How do the regulatory mechanisms work? What are the similarities and differences in these two types of organisms in terms of the specific components of the regulatory mechanisms?arrow_forwardIdentify the labeled factors in the figure below (A and B) and indicate the direction of transcription. ts A O A= Mediator, B= TFIID; direction is into the screen O A= TEIIE, B= TFIIH; direction is into the screen rences A= TBP, B= TEIIB; direction is out of the screen O A= TFIIF, B= TFIIE; direction is out of the screenarrow_forward
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