Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 12, Problem 7RQ
Summary Introduction

To review:

The important considerations for the construction of a plasmid synthesizing His6 (histidine)-tagged protein.

Introduction:

A circular double-stranded DNA (deoxyribonucleic acid) molecule that replicates independently is known as a plasmid. Plasmids exist naturally in bacterial cells to provide special benefits like antibiotic resistance. His6-tagged proteins can be identified by the presence of a string of histidine residues that can bind to nickel under suitable environment.

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