Essential Cell Biology 5e
Essential Cell Biology 5e
5th Edition
ISBN: 9780393691108
Author: Bruce Alberts, Karen Hopkin, Alexander D Johnson, David Morgan, Martin Raff, Keith Roberts, Peter Wa
Publisher: W. W. Norton
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Chapter 12, Problem 3Q

(A)

Summary Introduction

To explain: The channels of patch-clamp experiment in which electric current passes through the patch of membrane.

Introduction: Using the patch-clamp technique, the sympathetic ganglion cells are studied under nicotinic acetylcholine receptor ion channels. Micro superfusion of the ion channels facilitates the flow through the micro electrode that is sealed with the cell membrane. The synchronous membrane polarization and depolarization cause influx of ions, which results in fluctuation of the current generated in the sympathetic ganglion cells between -70mV and 50mV. Thus, the fluctuating current source is initiated by the nicotine action of acetylcholine receptor ion channels.

(B) (i)

Summary Introduction

To explain: What would be the effect on the recording if acetylcholine is omitted.

(B) (ii)

Summary Introduction

To explain: What would be the effect on the recording if acetylcholine is added to the solution outside the microelectrode.

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