MOLECULAR BIOLOGY OF THE CELL (LL)
MOLECULAR BIOLOGY OF THE CELL (LL)
6th Edition
ISBN: 9780393876109
Author: ALBERTS
Publisher: NORTON
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Chapter 12, Problem 3P
Summary Introduction

To examine: Whether the statement “To avoid the inevitable collisions that would occur if two-way traffic through a single pore were allowed, nuclear pore complexes are specialized so that some mediate import while others mediate export” is true or false.

Concept introduction: The nucleus is the site of replication, transcription, DNA repair and recombination. The nuclear membrane has nuclear pores surrounded by nucleoporins or nuclear pore complexes which facilitate the flow of RNA, proteins, ions and other molecules between cytoplasm and nucleoplasm.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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