EBK BIOLOGY
11th Edition
ISBN: 8220106820636
Author: Martin
Publisher: CENGAGE L
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Chapter 11.4, Problem 3C
Summary Introduction
To explain: The way in which examples of pleiotropy and epistasis differ in terms of the minimum number of loci required.
Introduction: A phenomenon where expression of one allele affects other independently inherited alleles is called epistasis. Pleiotropy is an ability of one gene to have multiple effects on
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 11 Solutions
EBK BIOLOGY
Ch. 11.1 - Define the terms phenotype, genotype, locus,...Ch. 11.1 - Describe Mendels principles of segregation and...Ch. 11.1 - Distinguish among monohybrid, dihybrid, and test...Ch. 11.1 - Explain Mendels principles of segregation and...Ch. 11.1 - What is the maximum number of different alleles...Ch. 11.1 - Can Mendels principle of segregation be...Ch. 11.1 - Prob. 3CCh. 11.2 - PREDICT Use the rules of probability to answer the...Ch. 11.2 - In answering the previous question, did you use...Ch. 11.3 - Define linkage and relate it to specific events in...
Ch. 11.3 - Show how data from a two-point test cross can be...Ch. 11.3 - Discuss the genetic determination of sex and the...Ch. 11.3 - What ratio of genotypes to phenotypes is observed...Ch. 11.3 - Prob. 2CCh. 11.3 - CONNECT Two loci exhibit 5% recombination between...Ch. 11.3 - Which chromosome determines the male sex in humans...Ch. 11.3 - Prob. 5CCh. 11.4 - Explain some of the ways genes may interact to...Ch. 11.4 - Distinguish among incomplete dominance,...Ch. 11.4 - Prob. 11LOCh. 11.4 - Prob. 1CCh. 11.4 - What is the difference between multiple alleles...Ch. 11.4 - Prob. 3CCh. 11.4 - Prob. 4CCh. 11 - One of the autosomal loci controlling eye color in...Ch. 11 - The F1 flies described in question 1 were mated...Ch. 11 - The type of cross described in question 2 is (a)...Ch. 11 - Individuals of genotype AaBb were crossed with...Ch. 11 - Assume that the ratio of females to males is 1:1....Ch. 11 - Redgreen color blindness is an X-linked recessive...Ch. 11 - When two long-winged flies were mated, the...Ch. 11 - The long hair of Persian cats is recessive to the...Ch. 11 - Mr. and Mrs. Smith are concerned because their own...Ch. 11 - A walnut comb rooster is mated to three hens. Hen...Ch. 11 - Individuals of genotype AaBb were mated to...Ch. 11 - Genes A and B are 6 map units apart, and A and C...Ch. 11 - VISUALIZE Sketch a series of diagrams showing each...Ch. 11 - Can you always ascertain an organisms genotype for...Ch. 11 - CONNECT Compare the mechanisms of genetic...Ch. 11 - EVOLUTION LINK Darwins theory of evolution by...Ch. 11 - INTERPRET DATA Using the graph in Figure 11-20,...
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