Biochemistry: The Molecular Basis of Life
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 11, Problem 3Q
Summary Introduction

To review:

The process of emulsification and description of the interaction of soap with an oil droplet along with a diagram.

Introduction:

Soaps are sodium or potassium salts of fatty acids that have an amphipathic structure. They have a hydrophilic head group (composed of the carboxyl group) and a hydrophobic tail (composed of the long hydrocarbon chain of the fatty acid).

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The carbon-nitrogen peptide bond is rigid, but rotation can take place about the bond between the nitrogen and a-carbon atoms and the bond between the a-carbon and the carbonyl carbon atom. These bond angles define the conformation of the peptide chain. Consider the Ramachandran plot shown to answer both questions. fr (degrees) 180 120 8280 60 ° -60 Which observation most likely describes part of an observed a helix? p=-59 degrees; y = +150 degrees; many Ser residues qp=-140 degrees; w +130 degrees; many Val residues qp=-57 degrees; y = -47 degrees; many Pro residues qp=-60 degrees; y = -47 degrees; many Ala residues p = +60 degrees; y = +40 degrees; many Gly residues Which observations would not likely occur in a ẞ sheet? qp=-59 degrees; y = -49 degrees; many Ala residues qp = +60 degrees; w = +60 degrees; many Gly residues qp=-120 degrees; y = +120 degrees; many Tyr residues ☐ ☐ qp=-139 degrees; y = +135 degrees; many Val residues op=-51 degrees; w+153 degrees; many Gly and Pro…
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A protein biochemist attempted to determine the amino acid sequence of a decapeptide. The results from the trypsin, chymotrypsin, and cyanogen bromide treatments are shown: ⚫ Trypsin digestion gave two fragments with multiple residues (not in order): • T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr o T2: Lys, Met, Val Chymotrypsin digestion gave four fragments with multiple residues (not in order): • CT1: Ala, Phe • CT2: Thr, Trp • CT3: Lys, Met, Tyr, Val 。 CT4: Arg, Gly ⚫ Treatment with cyanogen bromide yielded a single amino acid, methionine, and a nonapeptide. What is a possible sequence of the decapeptide? Use three-letter abbreviations in your answer, and add a dash between each residue.

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Biochemistry: The Molecular Basis of Life

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