Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 11, Problem 29P
A number of yeast-derived elements were added to the circular bacterial plasmid pBR322. Yeast that require uracil for growth (Ura- cells) were transformed with these modified plasmids and Ura+ colonies were selected by growth in media lacking uracil. For plasmids containing each of the elements listed in parts (a) to (c), indicate whether you expect the plasmid to integrate into a chromosome by recombination, or instead whether it is maintained separately as a plasmid. If the plasmid is maintained autonomously, is it stably inherited by all of the daughter cells of subsequent generations when you no longer select for Ura+ cells (that is, when you grow the yeast in media containing uracil)?
| a. | URA+ gene |
| b. | URA+ gene, ARS |
| c. | URA+ gene, ARS, CEN (centromere) |
| d. | What would need to be added in order for these sequences to be maintained stably in yeast cells as a linear artificial chromosome? |
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Chapter 11 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 11 - For each of the terms in the left column, choose...Ch. 11 - Many proteins other than histones are found...Ch. 11 - What difference is there in the compaction of...Ch. 11 - What is the role of the core histones in...Ch. 11 - a. About how many molecules of histone H2A would...Ch. 11 - The enzyme micrococcal nuclease can cleave...Ch. 11 - a. What letters are used to represent the short...Ch. 11 - About 2000 G bands are visible in a...Ch. 11 - Suppose you performed a fluorescence in situ...Ch. 11 - Which of the following would be suggested by a...
Ch. 11 - For each of the following pairs of chromatin...Ch. 11 - a. Drosophila b. Humans Give examples of...Ch. 11 - One histone modification that is seen consistently...Ch. 11 - Drosophila geneticists have isolated many...Ch. 11 - On the following figures, genes A and B are on the...Ch. 11 - Prob. 16PCh. 11 - The human genome contains about 3 billion base...Ch. 11 - Prob. 18PCh. 11 - Prob. 19PCh. 11 - Prob. 20PCh. 11 - Prob. 21PCh. 11 - If you are comparing the two telomeres in each...Ch. 11 - a. What DNA sequences are commonly found at human...Ch. 11 - Prob. 24PCh. 11 - Prob. 25PCh. 11 - Prob. 26PCh. 11 - Give at least one example of a chromosomal...Ch. 11 - a. Give at least three examples of types of...Ch. 11 - A number of yeast-derived elements were added to...Ch. 11 - Prob. 30P
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