Genetics: From Genes to Genomes, 5th edition
Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 11, Problem 21P
Summary Introduction

a.

To determine:

The results observed in the DNA containing multiple copies of 3′ AATCCC 5′ being used as a probe.

Introduction:

The chromosomes are condensed structures that are formed during the early phase of cell division from the loose network of chromatin thread and then regain their original structure after being divided into daughter cells.

Summary Introduction

b.

To determine:

The sequence of the probe to be used to track the end of the chromosome in a FISH experiment.

Introduction:

The genes are the sequence of nucleotides that are present on the chromosomes and encode for a specific protein that plays a crucial role in the functioning of the different processes in an organism. The gene is located at specific gene loci and can be structural or regulatory in nature.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
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