Concept explainers
If a restriction enzyme cuts between the G and the A whenever it encounters the sequence GAATTC, how many fragments will be produced when the enzyme is digested with DNA with the following sequence? TGAGAATTCAACTGAATTCAAATTCGAATTCTTAGC
- a. Two
- b. Three
- c. Four
- d. Five

Introduction:
Restriction enzymes are the enzymes that digest the higher molecular weight DNA in to smaller fragments. They are also known as restriction endonucleases.
Answer to Problem 1MCQ
Correct answer:
There are three sequences of GAATTC present on the DNA fragment and the fragments formed after the cleavage of the DNA are four in numbers. Therefore, option (c) is correct.
Explanation of Solution
Reason for correct statement:
Restriction endonucleases digest a double stranded DNA at specific sites. These specific sites are also called recognition site that present as palindrome. If the restriction enzyme given cuts between the G and A whenever it encounters the sequence GAATTC on the given DNA with the following sequence TGAGAATTCTGAATTCAAATTCGAATTCTTAGC, the cleaving of the DNA will be given as follows:
As shown, after the cleavage of the DNA by the help of the restriction enzyme, four fragments will be formed.
Option (c) is given as “Four”.
As, “the restriction enzyme recognizes three sequence on the given DNA, so it will make three cuts, that results in the production of the four DNA fragments,” is the right answer.
Hence, option (c) is correct.
Reasons for the incorrect statements:
Option (a), is given as “Two”.
The fragments of the DNA formed will be four after the action of the restriction enzyme. Hence, it is a wrong answer.
Option (b), is given as “Three”.
The DNA fragment formed after the cleavage will be four in numbers. Hence, it is a wrong answer.
Option (d), is given as “Five”.
For DNA fragments will be formed after the cutting action of the restriction enzyme at the given recognition site. Hence, it is a wrong answer.
Hence, options (a), (b), and (d), are incorrect.
Restriction enzymes are also known as molecular scissors that could cut double stranded DNA molecules at specific sites. It is an important tool that is used in the manipulation of DNA.
Want to see more full solutions like this?
Chapter 11 Solutions
BIOL:CONCEPT+INVEST.ETEXT
Additional Science Textbook Solutions
Physics for Scientists and Engineers
Introductory Chemistry (6th Edition)
Laboratory Manual For Human Anatomy & Physiology
Microbiology Fundamentals: A Clinical Approach
Human Physiology: An Integrated Approach (8th Edition)
- 30) A B CDEFG Refer to the accompanying figure. Which of the following forms a monophyletic group? A) A, B, C, and D B) C and D C) D, E, and F D) E, F, and Garrow_forwardMolecular Biology Question. Please help with step solution and explanation. Thank you: The Polymerase Chain Reaction (PCR) reaction consists of three steps denaturation, hybridization, and elongation. Please describe what occurs in the annealing step of the PCR reaction. (I think annealing step is hybridization). What are the other two steps of PCR, and what are their functions? Next, suppose the Tm for the two primers being used are 54C for Primer A and 67C for Primer B. Regarding annealing step temperature, I have the following choices for the temperature used during the annealing step:(a) 43C (b) 49C (c) 62C (d) 73C Which temperature/temperatures should I choose? What is the corresponding correct explanation, and why would I not use the other temperatures? Have a good day!arrow_forwardUsing the data provided on the mean body mass and horn size of 4-year-old male sheep, draw a scatterplot graph to examine how body mass and horn size changed over time.arrow_forward
- Please write a 500-word report about the intake of saturated fat, sodium, alcoholic beverages, or added sugar in America. Choose ONE of these and write about what is recommended by the Dietary Guidelines for Americans (guideline #4) and why Americans exceed the intake of that nutrient. Explain what we could do as a society and/or individuals to reduce our intake of your chosen nutrient.arrow_forwardWrite a 500-word report indicating how you can change the quantity or quality of TWO nutrients where your intake was LOWER than what is recommended by the Dietary Guidelines for Americans and/or the DRIs. Indicate how the lack of the nutrient may affect your health. For full credit, all of the following points must be addressed and elaborated on in more detail for each nutrient: The name of the nutrient At least 2 main functions of the nutrient (example: “Vitamin D regulates calcium levels in the blood and calcification of bones.”) Your percent intake compared to the RDA/DRI (example “I consumed 50% of the RDA for vitamin D”) Indicate why your intake was below the recommendations (example: “I only had one serving of dairy products and that was why I was below the recommendations for vitamin D”) How would you change your dietary pattern to meet the recommendations? – be sure to list specific foods (example: “I would add a yogurt and a glass of milk to each day in order to increase my…arrow_forwardWhy are nutrient absorption and dosage levels important when taking multivitamins and vitamin and mineral supplements?arrow_forward
- I'm struggling with this topic and would really appreciate your help. I need to hand-draw a diagram and explain the process of sexual differentiation in humans, including structures, hormones, enzymes, and other details. Could you also make sure to include these terms in the explanation? . Gonads . Wolffian ducts • Müllerian ducts . ⚫ Testes . Testosterone • Anti-Müllerian Hormone (AMH) . Epididymis • Vas deferens ⚫ Seminal vesicles ⚫ 5-alpha reductase ⚫ DHT - Penis . Scrotum . Ovaries • Uterus ⚫ Fallopian tubes - Vagina - Clitoris . Labia Thank you so much for your help!arrow_forwardRequisition Exercise A phlebotomist goes to a patient’s room with the following requisition. Hometown Hospital USA 125 Goodcare Avenue Small Town, USAarrow_forwardI’m struggling with this topic and would really appreciate your help. I need to hand-draw a diagram and explain the process of sexual differentiation in humans, including structures, hormones, enzymes, and other details. Could you also make sure to include these terms in the explanation? • Gonads • Wolffian ducts • Müllerian ducts • Testes • Testosterone • Anti-Müllerian Hormone (AMH) • Epididymis • Vas deferens • Seminal vesicles • 5-alpha reductase • DHT • Penis • Scrotum • Ovaries • Uterus • Fallopian tubes • Vagina • Clitoris • Labia Thank you so much for your help!arrow_forward
- I’m struggling with this topic and would really appreciate your help. I need to hand-draw a diagram and explain the process of sexual differentiation in humans, including structures, hormones, enzymes, and other details. Could you also make sure to include these terms in the explanation? • Gonads • Wolffian ducts • Müllerian ducts • Testes • Testosterone • Anti-Müllerian Hormone (AMH) • Epididymis • Vas deferens • Seminal vesicles • 5-alpha reductase • DHT • Penis • Scrotum • Ovaries • Uterus • Fallopian tubes • Vagina • Clitoris • Labia Thank you so much for your help!arrow_forwardOlder adults have unique challenges in terms of their nutrient needs and physiological changes. Some changes may make it difficult to consume a healthful diet, so it is important to identify strategies to help overcome these obstacles. From the list below, choose all the correct statements about changes in older adults. Select all that apply. Poor vision can make it difficult for older adults to get to a supermarket, and to prepare meals. With age, taste and visual perception decline. As people age, salivary production increases. In older adults with dysphagia, foods like creamy soups, applesauce, and yogurt are usually well tolerated. Lean body mass increases in older adults.arrow_forwardWhen physical activity increases, energy requirements increase also. Depending on the type, intensity, and duration of physical activity, the body’s requirements for certain macronutrients may change as well. From the list below, choose all the correct statements about the effects of increased physical activity or athletic training. Select all that apply. An athlete who weighs 70 kg (154 lb) should consume 420 to 700 g of carbohydrate per day. How much additional energy an athlete needs depends on the specific activity the athlete engages in and the frequency of the activity. Those participating in vigorous exercise should restrict their fat intake to less than 15%% of total energy intake. Athletes who are following energy-restricted diets are at risk for consuming insufficient protein. The recommendation to limit saturated fat intake to less than 10%% of total energy intake does not apply to athletes or those who regularly engage in vigorous physical activity.arrow_forward
- Concepts of BiologyBiologyISBN:9781938168116Author:Samantha Fowler, Rebecca Roush, James WisePublisher:OpenStax CollegeHuman Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
- Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage LearningHuman Biology (MindTap Course List)BiologyISBN:9781305112100Author:Cecie Starr, Beverly McMillanPublisher:Cengage LearningBiology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning





