a.
To determine:
The effects of mutation of regulatory gene in which repressor will not bind to lactose, mutation of operator in which repressor will not bind to operator and mutation of regulatory gene in which repressor will not bind to operator, RNA polymerase will not attach to promoter
Concept introduction:
Operon can be defined as a set of adjacent structural genes with adjacent regulatory signals that affect transcription of the structural genes. Each operon is consisted of structural genes, promoter. An operator is a DNA segment that turns the gene on and off. It interaction with proteins increases or decrease the rate of transcription from occurring thus make an operon inducible or repressible. And promoter is segment of DNA where RNA polymerase binds to begin the transcription protein. Lac operon is an inducible operon in which structural gene Z, Y, and A are induced in the presence of lactose.
b.
To determine:
The effect of mutation of operator in which repressor will not bind to operator.
Concept introduction:
Operon can be defined as a set of adjacent structural genes with adjacent regulatory signals that affect transcription of the structural genes. Each operon is consisted of structural genes, promoter. An operator is a DNA segment that turns the gene on and off. It interaction with proteins increases or decrease the rate of transcription from occurring thus make an operon inducible or repressible. And promoter is segment of DNA where RNA polymerase binds to begin the transcription protein. Lac operon is an inducible operon in which structural gene Z, Y, and A are induced in the presence of lactose.
c.
To determine:
The effect mutation of regulatory gene in which repressor will not bind to operator.
Concept introduction:
Operon can be defined as a set of adjacent structural genes with adjacent regulatory signals that affect transcription of the structural genes. Each operon is consisted of structural genes, promoter. An operator is a DNA segment that turns the gene on and off. It interaction with proteins increases or decrease the rate of transcription from occurring thus make an operon inducible or repressible. And promoter is segment of DNA where RNA polymerase binds to begin the transcription protein. Lac operon is an inducible operon in which structural gene Z, Y, and A are induced in the presence of lactose.
d.
To determine:
The effect mutation of regulatory gene if RNA polymerase will not attach to promoter.
Concept introduction:
Operon can be defined as a set of adjacent structural genes with adjacent regulatory signals that affect transcription of the structural genes. Each operon is consisted of structural genes, promoter. An operator is a DNA segment that turns the gene on and off. It interaction with proteins increases or decrease the rate of transcription from occurring thus make an operon inducible or repressible. And promoter is segment of DNA where RNA polymerase binds to begin the transcription protein. Lac operon is an inducible operon in which structural gene Z, Y, and A are induced in the presence of lactose.
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