Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 10.13, Problem 3MQ
Summary Introduction
Most of the prokaryotes like bacteria and Achaea have a characteristic genomic mechanism called CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats). This CRISPR is found in bacterial chromosomes. This CRISPR have constant repeats of variable DNA segments called spacers. These spacer DNA recognize the foreign DNA as it encounters each time and is stored in its memory bank.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 10 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 10.1 - Distinguish between a positive-strand RNA virus...Ch. 10.1 - Prob. 2MQCh. 10.1 - Prob. 3MQCh. 10.1 - Describe the classes of viruses based on their...Ch. 10.2 - Prob. 1MQCh. 10.2 - Prob. 2MQCh. 10.2 - Prob. 3MQCh. 10.2 - Prob. 1CRCh. 10.3 - Prob. 1MQCh. 10.3 - In the X174 genome, describe the difference...
Ch. 10.3 - Prob. 3MQCh. 10.3 - Describe how the genome of bacteriophage X174 is...Ch. 10.4 - In what major way does transcription of phage DNA...Ch. 10.4 - Prob. 2MQCh. 10.4 - Why can it be said that transcription of the...Ch. 10.5 - What type of genome is seen in most archaeal...Ch. 10.5 - Compared with other archaeal viruses, what are two...Ch. 10.5 - Prob. 1CRCh. 10.6 - Prob. 1MQCh. 10.6 - Prob. 2MQCh. 10.6 - Prob. 3MQCh. 10.6 - Prob. 1CRCh. 10.7 - Prob. 1MQCh. 10.7 - Prob. 2MQCh. 10.7 - Prob. 3MQCh. 10.7 - Prob. 1CRCh. 10.8 - Prob. 1MQCh. 10.8 - Prob. 2MQCh. 10.8 - How are protein synthesis and genomic replication...Ch. 10.8 - Prob. 1CRCh. 10.9 - Prob. 1MQCh. 10.9 - Prob. 2MQCh. 10.9 - Prob. 3MQCh. 10.9 - Rabies virus and poliovirus both have...Ch. 10.10 - Prob. 1MQCh. 10.10 - Prob. 2MQCh. 10.10 - Prob. 3MQCh. 10.10 - Prob. 1CRCh. 10.11 - Prob. 1MQCh. 10.11 - Prob. 2MQCh. 10.11 - How does the role of reverse transcriptase in the...Ch. 10.11 - Why do both hepadnaviruses and retroviruses...Ch. 10.12 - What type of bacteriophages are most common in the...Ch. 10.12 - Prob. 2MQCh. 10.12 - Prob. 3MQCh. 10.12 - Prob. 1CRCh. 10.13 - Prob. 1MQCh. 10.13 - Prob. 2MQCh. 10.13 - Prob. 3MQCh. 10.13 - Prob. 1CRCh. 10.14 - Prob. 1MQCh. 10.14 - Prob. 2MQCh. 10.14 - Prob. 3MQCh. 10.14 - How do bacterial viruses help prevent human...Ch. 10.15 - If viroids are circular molecules, why are they...Ch. 10.15 - Prob. 2MQCh. 10.15 - Prob. 1CRCh. 10.16 - Prob. 1MQCh. 10.16 - Prob. 2MQCh. 10.16 - Prob. 3MQCh. 10.16 - What are the similarities and differences between...Ch. 10 - Not all proteins are made from the RNA genome of...Ch. 10 - Replication of both strands of DNA in adenoviruses...Ch. 10 - Imagine that you are a researcher at a...Ch. 10 - Prob. 4AQ
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