Chapter 10, Problem 8Q

A.

Summary Introduction

To examine: Whether the statement “Restriction nucleases cut DNA at specific sites that are always located between genes” is correct or incorrect.

B.

Summary Introduction

To examine: Whether the statement “DNA migrates toward the positive electrode during electrophoresis” is correct or incorrect.

C.

Summary Introduction

To examine: Whether the statement “Clones isolated from cDNA libraries contain promoter sequences” is correct or incorrect.

D.

Summary Introduction

To explain: Whether the statement “PCR utilizes a heat stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured” is correct or incorrect.

E.

Summary Introduction

To examine: Whether the statement “Digestion of genomic DNA with Alu I, a restriction enzyme that recognizes a four-nucleotide sequence produces fragments that are all exactly 256 nucleotides in length” is correct or incorrect.

F.

Summary Introduction

To examine: Whether the statement “To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used” is correct or incorrect.

G.

Summary Introduction

To examine: Whether the statement “DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome” is correct or incorrect.

H.

Summary Introduction

To examine: Whether the statement “It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same” is correct or incorrect.