The sequence of DNA used in the given sequencing reaction. Introduction: Gel electrophoresis is an electrophoretic technique that is widely used for the separation of DNA, RNA, and proteins. Here, the sample that has to be separated is loaded at one end of the gel and forced to migrate to the other end of the gel by applying the electric current . The molecules migrate across the cell according to their size and charge. DNA fragments are loaded in the wells after staining it by suitable DNA-binding dye. After the electrophoretic run, the bands can be visualized under UV-transilluminator. From the position of bands, the sequence of the any given DNA fragments can be determined.

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Chapter 10, Problem 9Q

A.

Summary Introduction

To determine: The sequence of DNA used in the given sequencing reaction.

Introduction: Gel electrophoresis is an electrophoretic technique that is widely used for the separation of DNA, RNA, and proteins. Here, the sample that has to be separated is loaded at one end of the gel and forced to migrate to the other end of the gel by applying the electric current. The molecules migrate across the cell according to their size and charge. DNA fragments are loaded in the wells after staining it by suitable DNA-binding dye. After the electrophoretic run, the bands can be visualized under UV-transilluminator. From the position of bands, the sequence of the any given DNA fragments can be determined.

B.

Summary Introduction

To determine: The amino acid sequence of the given protein sequence.

Introduction: The central dogma goes by the following process, namely replication, transcription, and translation. If the DNA sequence is known then the mRNA sequence can be determined. The amino acid sequence and protein sequence can be determined using the degenerate genetic code.

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