Whether it is possible to isolate ice-protein mutant bacteria without the help of modern DNA technology. Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

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Answer 1

Question

Chapter 10, Problem 13Q

Summary Introduction

To explain: Whether it is possible to isolate ice-protein mutant bacteria without the help of modern DNA technology.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

Summary Introduction

To explain: The possibility that such mutant bacteria have already occurred in nature.

Introduction: Recombinant DNA molecule forms by genetic recombination. It contains foreign DNA sequence from multiple sources. A recombinant DNA molecule (such as plasmid containing foreign DNA insert) can be grown in culture condition.

Summary Introduction

To explain: Whether the mutant bacterial strains isolated from nature would be of lesser concern than the mutant bacteria produced by recombinant DNA techniques.

Introduction: Recombinant DNA technology in order to produce novel genetic combinations combines the genes from two different organisms and introduces them inside a host. Generally, the genes of eukaryotic organisms of higher order are inserted into plasmid vectors and then are inserted into microorganisms.

Summary Introduction

To explain: Whether we should be concerned about the risk posed by the application of recombinant DNA techniques in agriculture and medicine.

Introduction: In the agricultural field, recombinant DNA technology is used to increase plant resistance to disease. This DNA technique is also important for paharmaceutical industry and medicine. This helps to diagnose genetic disease, such as Hungitons’s disease, and sickle-cell disease.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

Question #5: Assume that two genes are identified that confer gametophytic facultative apomixis in soybean. The genes show independent assortment. Recessive alleles at both loci are required for the facultative apomixis. Facultative apomixis is triggered when the temperature at pollination is above 20 degrees C. At temperatures below 20 degrees C, all reproduction is sexual, independent of genotype. A facultative apomict male, capable of producing viable pollen, was crossed with a sexually reproducing female. Assuming the parents are completely inbred, what are the predicted phenotypic ratios (apomict: non-apomict) for the F1, F2, and DH (F1-derived) generations at each of the following temperatures*: a) 15°C? b) 25°C? *for full credit, show crosses and genotypes where appropriate. Remember to position the female first (left side) in the cross. Type your answer here:

Answer 2

Question

Chapter 10, Problem 13Q

Summary Introduction

To explain: Whether it is possible to isolate ice-protein mutant bacteria without the help of modern DNA technology.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

Summary Introduction

To explain: The possibility that such mutant bacteria have already occurred in nature.

Introduction: Recombinant DNA molecule forms by genetic recombination. It contains foreign DNA sequence from multiple sources. A recombinant DNA molecule (such as plasmid containing foreign DNA insert) can be grown in culture condition.

Summary Introduction

To explain: Whether the mutant bacterial strains isolated from nature would be of lesser concern than the mutant bacteria produced by recombinant DNA techniques.

Introduction: Recombinant DNA technology in order to produce novel genetic combinations combines the genes from two different organisms and introduces them inside a host. Generally, the genes of eukaryotic organisms of higher order are inserted into plasmid vectors and then are inserted into microorganisms.

Summary Introduction

To explain: Whether we should be concerned about the risk posed by the application of recombinant DNA techniques in agriculture and medicine.

Introduction: In the agricultural field, recombinant DNA technology is used to increase plant resistance to disease. This DNA technique is also important for paharmaceutical industry and medicine. This helps to diagnose genetic disease, such as Hungitons’s disease, and sickle-cell disease.

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Answer 3

Question

Chapter 10, Problem 13Q

Summary Introduction

To explain: Whether it is possible to isolate ice-protein mutant bacteria without the help of modern DNA technology.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

Summary Introduction

To explain: The possibility that such mutant bacteria have already occurred in nature.

Introduction: Recombinant DNA molecule forms by genetic recombination. It contains foreign DNA sequence from multiple sources. A recombinant DNA molecule (such as plasmid containing foreign DNA insert) can be grown in culture condition.

Summary Introduction

To explain: Whether the mutant bacterial strains isolated from nature would be of lesser concern than the mutant bacteria produced by recombinant DNA techniques.

Introduction: Recombinant DNA technology in order to produce novel genetic combinations combines the genes from two different organisms and introduces them inside a host. Generally, the genes of eukaryotic organisms of higher order are inserted into plasmid vectors and then are inserted into microorganisms.

Summary Introduction

To explain: Whether we should be concerned about the risk posed by the application of recombinant DNA techniques in agriculture and medicine.

Introduction: In the agricultural field, recombinant DNA technology is used to increase plant resistance to disease. This DNA technique is also important for paharmaceutical industry and medicine. This helps to diagnose genetic disease, such as Hungitons’s disease, and sickle-cell disease.

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Answer 4

Question

Chapter 10, Problem 13Q

Summary Introduction

To explain: Whether it is possible to isolate ice-protein mutant bacteria without the help of modern DNA technology.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

Summary Introduction

To explain: The possibility that such mutant bacteria have already occurred in nature.

Introduction: Recombinant DNA molecule forms by genetic recombination. It contains foreign DNA sequence from multiple sources. A recombinant DNA molecule (such as plasmid containing foreign DNA insert) can be grown in culture condition.

Summary Introduction

To explain: Whether the mutant bacterial strains isolated from nature would be of lesser concern than the mutant bacteria produced by recombinant DNA techniques.

Introduction: Recombinant DNA technology in order to produce novel genetic combinations combines the genes from two different organisms and introduces them inside a host. Generally, the genes of eukaryotic organisms of higher order are inserted into plasmid vectors and then are inserted into microorganisms.

Summary Introduction

To explain: Whether we should be concerned about the risk posed by the application of recombinant DNA techniques in agriculture and medicine.

Introduction: In the agricultural field, recombinant DNA technology is used to increase plant resistance to disease. This DNA technique is also important for paharmaceutical industry and medicine. This helps to diagnose genetic disease, such as Hungitons’s disease, and sickle-cell disease.

Expert Solution & Answer

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Answer 5

Chapter 10, Problem 13Q

Summary Introduction

To explain: Whether it is possible to isolate ice-protein mutant bacteria without the help of modern DNA technology.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

Summary Introduction

To explain: The possibility that such mutant bacteria have already occurred in nature.

Introduction: Recombinant DNA molecule forms by genetic recombination. It contains foreign DNA sequence from multiple sources. A recombinant DNA molecule (such as plasmid containing foreign DNA insert) can be grown in culture condition.

Summary Introduction

To explain: Whether the mutant bacterial strains isolated from nature would be of lesser concern than the mutant bacteria produced by recombinant DNA techniques.

Introduction: Recombinant DNA technology in order to produce novel genetic combinations combines the genes from two different organisms and introduces them inside a host. Generally, the genes of eukaryotic organisms of higher order are inserted into plasmid vectors and then are inserted into microorganisms.

Summary Introduction

To explain: Whether we should be concerned about the risk posed by the application of recombinant DNA techniques in agriculture and medicine.

Introduction: In the agricultural field, recombinant DNA technology is used to increase plant resistance to disease. This DNA technique is also important for paharmaceutical industry and medicine. This helps to diagnose genetic disease, such as Hungitons’s disease, and sickle-cell disease.

Answer 6

Chapter 10, Problem 13Q

Summary Introduction

To explain: Whether it is possible to isolate ice-protein mutant bacteria without the help of modern DNA technology.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

Answer 7

Chapter 10, Problem 13Q

Summary Introduction

To explain: Whether it is possible to isolate ice-protein mutant bacteria without the help of modern DNA technology.

Introduction: Recombinant DNA techniques are used for producing genetically modified organisms. It is also possible to integrate green fluorescent protein as molecular tag that allows its tracking inside the cell. The candidate gene can be knocked out to produce strain of desirable characteristics.

Answer 8

Summary Introduction

To explain: The possibility that such mutant bacteria have already occurred in nature.

Introduction: Recombinant DNA molecule forms by genetic recombination. It contains foreign DNA sequence from multiple sources. A recombinant DNA molecule (such as plasmid containing foreign DNA insert) can be grown in culture condition.

Answer 9

Summary Introduction

To explain: The possibility that such mutant bacteria have already occurred in nature.

Introduction: Recombinant DNA molecule forms by genetic recombination. It contains foreign DNA sequence from multiple sources. A recombinant DNA molecule (such as plasmid containing foreign DNA insert) can be grown in culture condition.

Answer 10

Summary Introduction

To explain: Whether the mutant bacterial strains isolated from nature would be of lesser concern than the mutant bacteria produced by recombinant DNA techniques.

Introduction: Recombinant DNA technology in order to produce novel genetic combinations combines the genes from two different organisms and introduces them inside a host. Generally, the genes of eukaryotic organisms of higher order are inserted into plasmid vectors and then are inserted into microorganisms.

Answer 11

Summary Introduction

To explain: Whether the mutant bacterial strains isolated from nature would be of lesser concern than the mutant bacteria produced by recombinant DNA techniques.

Introduction: Recombinant DNA technology in order to produce novel genetic combinations combines the genes from two different organisms and introduces them inside a host. Generally, the genes of eukaryotic organisms of higher order are inserted into plasmid vectors and then are inserted into microorganisms.

Answer 12

Summary Introduction

To explain: Whether we should be concerned about the risk posed by the application of recombinant DNA techniques in agriculture and medicine.

Introduction: In the agricultural field, recombinant DNA technology is used to increase plant resistance to disease. This DNA technique is also important for paharmaceutical industry and medicine. This helps to diagnose genetic disease, such as Hungitons’s disease, and sickle-cell disease.

Answer 13

Summary Introduction

To explain: Whether we should be concerned about the risk posed by the application of recombinant DNA techniques in agriculture and medicine.

Introduction: In the agricultural field, recombinant DNA technology is used to increase plant resistance to disease. This DNA technique is also important for paharmaceutical industry and medicine. This helps to diagnose genetic disease, such as Hungitons’s disease, and sickle-cell disease.

Answer 14

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Answer 17

Ch. 10 - Prob. 1Q Ch. 10 - Prob. 2Q Ch. 10 - Prob. 3Q Ch. 10 - Prob. 4Q Ch. 10 - Prob. 5Q Ch. 10 - Prob. 6Q Ch. 10 - Prob. 7Q Ch. 10 - Prob. 8Q Ch. 10 - Prob. 9Q Ch. 10 - Prob. 10Q

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Chapter 10 Solutions