a. To determine: The sequence that is genomic fragment and the sequence that is cDNA fragment. Introduction: The short sequences of DNA that are obtained from the genome of an organism are termed as genomic fragments. The fragments of complementary DNA that are stored in the cDNA library are termed as cDNA fragments.

Question

19. On the diagram below a. Label the three pictures as: DNA; polypeptide; or RNA. b. Label the arrows as: translation or transcription/RNA processing. c. Add the following details to the diagram. Promoter region TATA box Transcription start site Transcription terminator Intron (A,B,C,D) Exons (1,2,3,4,5) Splice sites 5' cap 5' UTR (untranslated region) 3' poly A tail 3' UTR (untranslated region) Translational start (AUG) Translational stop (UGA, UAG, or UAA) N and C ends of polypeptide 0000

Answer 1

Question

Chapter 10, Problem 5P

Summary Introduction

a.

To determine:

The sequence that is genomic fragment and the sequence that is cDNA fragment.

Introduction:

The short sequences of DNA that are obtained from the genome of an organism are termed as genomic fragments. The fragments of complementary DNA that are stored in the cDNA library are termed as cDNA fragments.

Summary Introduction

b.

To determine:

The RNA like strand of the genomic fragment and to represent vertical lines between exon and introns.

Introduction:

The genetic material of eukaryotes is composed of two parts. These are introns and exons. The coding parts of DNA that encode for amino acids are termed as exons. However, the non-coding parts that do not encode for amino acids are termed as introns. The prokaryotes are composed of only the exons. They do not contain introns.

Summary Introduction

c.

To determine:

The sequence features important for RNA splicing that is missing.

Introduction:

The mRNA contains both introns and exons. The exons encode for the amino acids while the introns do not encode for amino acids. They introns inhibit the synthesis of proteins. The process by which all introns are removed from mRNA is termed as RNA splicing.

Summary Introduction

d.

To determine:

The amino acid sequence of protein product of the gene.

Introduction:

The mRNA consists of many bases. A collection of three bases that has the capability to code for a particular amino acid is called codon. Codons are present in the mRNA. These codons attach with the anticodon part of tRNA to synthesize amino acid. The anticodon part of tRNA is complementary to the codon part of mRNA. As a result, these two join together and undergo a translation process to produce amino acids.

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19. On the diagram below a. Label the three pictures as: DNA; polypeptide; or RNA. b. Label the arrows as: translation or transcription/RNA processing. c. Add the following details to the diagram. Promoter region TATA box Transcription start site Transcription terminator Intron (A,B,C,D) Exons (1,2,3,4,5) Splice sites 5' cap 5' UTR (untranslated region) 3' poly A tail 3' UTR (untranslated region) Translational start (AUG) Translational stop (UGA, UAG, or UAA) N and C ends of polypeptide 0000

Match the letter labels in the figure below to the terms. Some letter labels are not used. MNNNNNNIN M C B A M D F E H K G 8

The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions. 1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction? 2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe: a) What molecule do you recommend for the trial. Why? b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for? 3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale. 4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale. Mutation 1 Mutation 2

Answer 2

Question

Chapter 10, Problem 5P

Summary Introduction

a.

To determine:

The sequence that is genomic fragment and the sequence that is cDNA fragment.

Introduction:

The short sequences of DNA that are obtained from the genome of an organism are termed as genomic fragments. The fragments of complementary DNA that are stored in the cDNA library are termed as cDNA fragments.

Summary Introduction

b.

To determine:

The RNA like strand of the genomic fragment and to represent vertical lines between exon and introns.

Introduction:

The genetic material of eukaryotes is composed of two parts. These are introns and exons. The coding parts of DNA that encode for amino acids are termed as exons. However, the non-coding parts that do not encode for amino acids are termed as introns. The prokaryotes are composed of only the exons. They do not contain introns.

Summary Introduction

c.

To determine:

The sequence features important for RNA splicing that is missing.

Introduction:

The mRNA contains both introns and exons. The exons encode for the amino acids while the introns do not encode for amino acids. They introns inhibit the synthesis of proteins. The process by which all introns are removed from mRNA is termed as RNA splicing.

Summary Introduction

d.

To determine:

The amino acid sequence of protein product of the gene.

Introduction:

The mRNA consists of many bases. A collection of three bases that has the capability to code for a particular amino acid is called codon. Codons are present in the mRNA. These codons attach with the anticodon part of tRNA to synthesize amino acid. The anticodon part of tRNA is complementary to the codon part of mRNA. As a result, these two join together and undergo a translation process to produce amino acids.

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Answer 3

Question

Chapter 10, Problem 5P

Summary Introduction

a.

To determine:

The sequence that is genomic fragment and the sequence that is cDNA fragment.

Introduction:

The short sequences of DNA that are obtained from the genome of an organism are termed as genomic fragments. The fragments of complementary DNA that are stored in the cDNA library are termed as cDNA fragments.

Summary Introduction

b.

To determine:

The RNA like strand of the genomic fragment and to represent vertical lines between exon and introns.

Introduction:

The genetic material of eukaryotes is composed of two parts. These are introns and exons. The coding parts of DNA that encode for amino acids are termed as exons. However, the non-coding parts that do not encode for amino acids are termed as introns. The prokaryotes are composed of only the exons. They do not contain introns.

Summary Introduction

c.

To determine:

The sequence features important for RNA splicing that is missing.

Introduction:

The mRNA contains both introns and exons. The exons encode for the amino acids while the introns do not encode for amino acids. They introns inhibit the synthesis of proteins. The process by which all introns are removed from mRNA is termed as RNA splicing.

Summary Introduction

d.

To determine:

The amino acid sequence of protein product of the gene.

Introduction:

The mRNA consists of many bases. A collection of three bases that has the capability to code for a particular amino acid is called codon. Codons are present in the mRNA. These codons attach with the anticodon part of tRNA to synthesize amino acid. The anticodon part of tRNA is complementary to the codon part of mRNA. As a result, these two join together and undergo a translation process to produce amino acids.

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Answer 4

Question

Chapter 10, Problem 5P

Summary Introduction

a.

To determine:

The sequence that is genomic fragment and the sequence that is cDNA fragment.

Introduction:

The short sequences of DNA that are obtained from the genome of an organism are termed as genomic fragments. The fragments of complementary DNA that are stored in the cDNA library are termed as cDNA fragments.

Summary Introduction

b.

To determine:

The RNA like strand of the genomic fragment and to represent vertical lines between exon and introns.

Introduction:

The genetic material of eukaryotes is composed of two parts. These are introns and exons. The coding parts of DNA that encode for amino acids are termed as exons. However, the non-coding parts that do not encode for amino acids are termed as introns. The prokaryotes are composed of only the exons. They do not contain introns.

Summary Introduction

c.

To determine:

The sequence features important for RNA splicing that is missing.

Introduction:

The mRNA contains both introns and exons. The exons encode for the amino acids while the introns do not encode for amino acids. They introns inhibit the synthesis of proteins. The process by which all introns are removed from mRNA is termed as RNA splicing.

Summary Introduction

d.

To determine:

The amino acid sequence of protein product of the gene.

Introduction:

The mRNA consists of many bases. A collection of three bases that has the capability to code for a particular amino acid is called codon. Codons are present in the mRNA. These codons attach with the anticodon part of tRNA to synthesize amino acid. The anticodon part of tRNA is complementary to the codon part of mRNA. As a result, these two join together and undergo a translation process to produce amino acids.

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Answer 5

Chapter 10, Problem 5P

Summary Introduction

a.

To determine:

The sequence that is genomic fragment and the sequence that is cDNA fragment.

Introduction:

The short sequences of DNA that are obtained from the genome of an organism are termed as genomic fragments. The fragments of complementary DNA that are stored in the cDNA library are termed as cDNA fragments.

Summary Introduction

b.

To determine:

The RNA like strand of the genomic fragment and to represent vertical lines between exon and introns.

Introduction:

The genetic material of eukaryotes is composed of two parts. These are introns and exons. The coding parts of DNA that encode for amino acids are termed as exons. However, the non-coding parts that do not encode for amino acids are termed as introns. The prokaryotes are composed of only the exons. They do not contain introns.

Summary Introduction

c.

To determine:

The sequence features important for RNA splicing that is missing.

Introduction:

The mRNA contains both introns and exons. The exons encode for the amino acids while the introns do not encode for amino acids. They introns inhibit the synthesis of proteins. The process by which all introns are removed from mRNA is termed as RNA splicing.

Summary Introduction

d.

To determine:

The amino acid sequence of protein product of the gene.

Introduction:

The mRNA consists of many bases. A collection of three bases that has the capability to code for a particular amino acid is called codon. Codons are present in the mRNA. These codons attach with the anticodon part of tRNA to synthesize amino acid. The anticodon part of tRNA is complementary to the codon part of mRNA. As a result, these two join together and undergo a translation process to produce amino acids.

Answer 6

Chapter 10, Problem 5P

Summary Introduction

a.

To determine:

The sequence that is genomic fragment and the sequence that is cDNA fragment.

Introduction:

The short sequences of DNA that are obtained from the genome of an organism are termed as genomic fragments. The fragments of complementary DNA that are stored in the cDNA library are termed as cDNA fragments.

Answer 7

Chapter 10, Problem 5P

Summary Introduction

a.

To determine:

The sequence that is genomic fragment and the sequence that is cDNA fragment.

Introduction:

The short sequences of DNA that are obtained from the genome of an organism are termed as genomic fragments. The fragments of complementary DNA that are stored in the cDNA library are termed as cDNA fragments.

Answer 8

Summary Introduction

b.

To determine:

The RNA like strand of the genomic fragment and to represent vertical lines between exon and introns.

Introduction:

The genetic material of eukaryotes is composed of two parts. These are introns and exons. The coding parts of DNA that encode for amino acids are termed as exons. However, the non-coding parts that do not encode for amino acids are termed as introns. The prokaryotes are composed of only the exons. They do not contain introns.

Answer 9

Summary Introduction

b.

To determine:

The RNA like strand of the genomic fragment and to represent vertical lines between exon and introns.

Introduction:

The genetic material of eukaryotes is composed of two parts. These are introns and exons. The coding parts of DNA that encode for amino acids are termed as exons. However, the non-coding parts that do not encode for amino acids are termed as introns. The prokaryotes are composed of only the exons. They do not contain introns.

Answer 10

Summary Introduction

c.

To determine:

The sequence features important for RNA splicing that is missing.

Introduction:

The mRNA contains both introns and exons. The exons encode for the amino acids while the introns do not encode for amino acids. They introns inhibit the synthesis of proteins. The process by which all introns are removed from mRNA is termed as RNA splicing.

Answer 11

Summary Introduction

c.

To determine:

The sequence features important for RNA splicing that is missing.

Introduction:

The mRNA contains both introns and exons. The exons encode for the amino acids while the introns do not encode for amino acids. They introns inhibit the synthesis of proteins. The process by which all introns are removed from mRNA is termed as RNA splicing.

Answer 12

Summary Introduction

d.

To determine:

The amino acid sequence of protein product of the gene.

Introduction:

The mRNA consists of many bases. A collection of three bases that has the capability to code for a particular amino acid is called codon. Codons are present in the mRNA. These codons attach with the anticodon part of tRNA to synthesize amino acid. The anticodon part of tRNA is complementary to the codon part of mRNA. As a result, these two join together and undergo a translation process to produce amino acids.

Answer 13

Summary Introduction

d.

To determine:

The amino acid sequence of protein product of the gene.

Introduction:

The mRNA consists of many bases. A collection of three bases that has the capability to code for a particular amino acid is called codon. Codons are present in the mRNA. These codons attach with the anticodon part of tRNA to synthesize amino acid. The anticodon part of tRNA is complementary to the codon part of mRNA. As a result, these two join together and undergo a translation process to produce amino acids.

Answer 14

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Ch. 10 - Prob. 1P Ch. 10 - List three independent techniques you could use to...Ch. 10 - Figure 10.2a has numbers indicating the...Ch. 10 - Which of the enzymes from the following list would...Ch. 10 - Prob. 5P Ch. 10 - a. What sequence information about a gene is...Ch. 10 - Why do geneticists studying eukaryotic organisms...Ch. 10 - Consider three different kinds of human libraries:...Ch. 10 - The human genome has been sequenced, but we still...Ch. 10 - This problem investigates issues encountered in...

Solution Summary: The author explains that the genomic and cDNA fragments are longer than the eukaryotes' genomes. The RNA polymerase is the enzyme that converts the double-stranded DNA into a

Chapter 10 Solutions