Concepts of Genetics (11th Edition)
Concepts of Genetics (11th Edition)
11th Edition
ISBN: 9780321948915
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 10, Problem 1NST

Would an experiment similar to that performed by Hershey and Chase work if the basic design were applied to the phenomenon of transformation? Explain why or why not.

Expert Solution & Answer
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Summary Introduction

To determine: The explanation of whether the design of Hershey and Chase work can be applied to the phenomenon of transformation.

Introduction: Bacteriophage T2 virus consists of a protein coat surrounding a core of DNA. Electron micrographs reveal the external structure of bacteriophage. It is composed of an icosahedral head plus a tail.

Explanation of Solution

The Hershey and Chase, experiment labeled deoxyribonucleic acids (DNA) or protein in a Phage T2 virus by mixing the phage in E.coli with either radioactive phosphorus or sulfur. The radioactive phages were then transferred to bacteria without radioactive elements. The progeny from this reproduction were labeled with radioactive phosphorus (label DNA) and not sulfur. The experiment concluded that DNA was passed on to the progeny cells and not the protein.

If Hershey and Chase's protocol was used during transformation in bacteria, the result would be the same. In theory, the general design would be appropriate, that some substance if labeled, would show up in the progeny of transformed bacteria. The transforming DNA amount is extremely small as compared with the recipient bacterium genomic DNA and its progeny. Assaying of labeled nucleic acid would be technically difficult in this protocol.

Conclusion

Thus, if the design of Hershey and Chase was applied to the phenomenon of transformation, the result would be the same.

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Chapter 10 Solutions

Concepts of Genetics (11th Edition)

Ch. 10 - Prob. 4PDQCh. 10 - When Avery and his colleagues had obtained what...Ch. 10 - Why were 32P and 35S chosen for use in the...Ch. 10 - Does the design of the HersheyChase experiment...Ch. 10 - What observations are consistent with the...Ch. 10 - What are the exceptions to the general rule that...Ch. 10 - Draw the chemical structure of the three...Ch. 10 - How are the carbon and nitrogen atoms of the...Ch. 10 - Adenine may also be named 6-amino purine. How...Ch. 10 - Draw the chemical structure of a dinucleotide...Ch. 10 - Describe the various characteristics of the...Ch. 10 - What evidence did Watson and Crick have at their...Ch. 10 - What might Watson and Crick have concluded had...Ch. 10 - How do covalent bonds differ from hydrogen bonds?...Ch. 10 - List three main differences between DNA and RNA.Ch. 10 - What are the three major types of RNA molecules?...Ch. 10 - Prob. 20PDQCh. 10 - What is the physical state of DNA after it is...Ch. 10 - What is the hyperchromic effect? How is it...Ch. 10 - Why is Tm related to base composition?Ch. 10 - What is the chemical basis of molecular...Ch. 10 - What did the WatsonCrick model suggest about the...Ch. 10 - A genetics student was asked to draw the chemical...Ch. 10 - Considering the information in this chapter on B-...Ch. 10 - One of the most common spontaneous lesions that...Ch. 10 - In some organisms, cytosine is methylated at...Ch. 10 - Because of its rapid turnaround time, fluorescent...Ch. 10 - Prob. 31PDQCh. 10 - Prob. 32ESPCh. 10 - Newsdate: March 1, 2030. A unique creature has...Ch. 10 - Prob. 34PDQCh. 10 - During gel electrophoresis, DNA molecules can...Ch. 10 - Electrophoresis is an extremely useful procedure...Ch. 10 - Following is a table (modified from Kropinski,...
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