Microbiology: Principles and Explorations
Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Chapter 1, Problem 3CTQ

As often happens in science, one observation or experiment that is used to look at one aspect or subdiscipline of science can lead to profound explanations or solutions in another aspect or discipline of science. Serendipity and experimental mistakes also play a role. Explain how this might apply to Angelina Hesse and the success of Robert Koch’s pure cultures. Louis Pasteur’s assistant and the success of the immunizations of chickens against chicken cholera, plus Alexander Fleming and Penicillium.

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Robert Koch developed a set of criteria (postulates) for conclusively demonstrating the aetiology (specific cause) of an infectious disease. Which of the following is not a postulate?     The infectious agent must be isolated and cultured in vitro     The disease is reproduced when a pure culture of the infectious agent is inoculated into a new susceptible host     The infectious agent can be recovered from the experimentally-infected host     The infectious agent is present in most cases of the disease
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is a procedure you could do, involving making a slide of the organism?

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Microbiology: Principles and Explorations

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cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY