EBK CAMPBELL BIOLOGY
10th Edition
ISBN: 9780136539414
Author: Reece
Publisher: VST
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Chapter 1, Problem 11TYU
DRAW IT With rough sketches, draw a biological hierarchy similar to the one in Figure 1.3 but using a coral reef as the ecosystem, a fish as the organism, its stomach as the organ, and DNA as the molecule. Include all levels in the hierarchy.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 1 Solutions
EBK CAMPBELL BIOLOGY
Ch. 1.1 - Starting with the molecular level in Figure 1.3,...Ch. 1.1 - Identify the theme or themes exemplified by (a)...Ch. 1.1 - WHAT IF? For each theme discussed in this...Ch. 1.2 - Prob. 1CCCh. 1.2 - Explain why "editing" is a metaphor for how...Ch. 1.2 - DRAW IT The three domains you learned about in...Ch. 1.3 - Contrast inductive reasoning with deductive...Ch. 1.3 - Prob. 2CCCh. 1.3 - Why is natural selection called a theory?Ch. 1.3 - WHAT IF? In the deserts of New Mexico, the soils...
Ch. 1.4 - How does science differ from technology?Ch. 1.4 - MAKE CONNECTIONS The gene that causes sickle-cell...Ch. 1 - Prob. 1.1CRCh. 1 - Prob. 1.2CRCh. 1 - What are the roles of gathering and interpreting...Ch. 1 - Explain why different approaches and diverse...Ch. 1 - Prob. 1TYUCh. 1 - Prob. 2TYUCh. 1 - Prob. 3TYUCh. 1 - Prob. 4TYUCh. 1 - Prob. 5TYUCh. 1 - Which of the following best demonstrates the unity...Ch. 1 - Prob. 7TYUCh. 1 - Which of the following statements best...Ch. 1 - Which of the following is an example of...Ch. 1 - Which sentence best describes the logic of...Ch. 1 - DRAW IT With rough sketches, draw a biological...Ch. 1 - EVOLUTION CONNECTION A typical prokaryotic cell...Ch. 1 - SCIENTIFIC INQUIRY Based an the results of the...Ch. 1 - WRITE ABOUT A THEME: EVOLUTION In a Short essay...Ch. 1 - SYNTHESIZE YOUR KNOWLEDGE Can you pick out the...
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