You use 0.1 ml of the stock of virus which you titrated in question #5 to infect 1 million cells. What is the MOI?
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You use 0.1 ml of the stock of virus which you titrated in question #5 to infect 1 million cells. What is the MOI?
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- Write down the name of the infectious agent of COVID-19 disease. What is the genetic material of the virus that causes COVID-19 disease?The herpes virus is a very large family of viruses which can cause human infections. There are many varioustypes which can cause different types of effects. Please discuss how you would determine which type of virus is causing the infection. Make sure to include 2-3 examples and how this will help differentiate between each.Jerome Vinograd found that when circular DNA from apolyoma virus is subjected to cesium chloride gradientcentrifugation, it separates into two distinct bands, oneconsisting of supercoiled DNA and the other of relaxedDNA. Explain how you would identify each band.
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- What percent of guanine exist in virus genome if it contained 35% if adenine in double stranded RNA genome after isolating it from a virus?2.4 SARS-CoV-2 is mainly transmitted through respiratory droplets when an infected person sneezes, coughs, or talks. How might this relate to the size and/or structure of viruses?Viruses are made up of the same biomolecules as cellular organisms (DNA, RNA, proteins, lipids, etc.), which are synthesized through enzymatic dehydration reactions from building blocks and disassembled by enzymatic hydrolysis. They infect cells and replicate themselves using resources from the host cells. Scientists discovered a previously unknown strain of virus in the tropical Pacific Ocean and it can infect higher-order eukaryotes. Initial analysis revealed that the virus is mainly composed of nucleic acids, proteins, and lipids. Chemical analysis after complete hydrolysis revealed the following building blocks (by weight): Chemical components Nucleosides Amino acids Lipids, phosphates, and carbohydrates Percentage by weight 20 30 50 Analysis of percentages of nucleosides yielded the following results (by molar ratio): Nucleosides A G T C U Percentage by mole 19 29 19 29 4 Which of the following events mostly involves hydrolysis reaction after the virus infects…