You identify an auxotroph that doesn't grow in normal medium, but does grow in medium supplemented with Q, indicating that it has a defect in one or more enzymes in this pathway. This auxotroph is also able to grow if you supplement the medium with D. Does this data support the one gene-one enzyme hypothesis? a yes, because only one enzyme is disrupted by the mutation b yes, because only one enzyme in the pathway is functional in the mutant strain c no, because multiple enzymes in the pathway could be functional in the mutant strain d no, because multiple enzymes could be disrupted by the mutation

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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You identify an auxotroph that doesn't grow in normal medium, but does grow in medium supplemented with Q, indicating that it has a defect in one or more enzymes in this pathway. This auxotroph is also able to grow if you supplement the medium with D. Does this data support the one gene-one enzyme hypothesis?

a yes, because only one enzyme is disrupted by the mutation
b

yes, because only one enzyme in the pathway is functional in the mutant strain

c

no, because multiple enzymes in the pathway could be functional in the mutant strain

d

no, because multiple enzymes could be disrupted by the mutation

E1 E2
ЕЗ
E4
E5
X— Ү — Z —
Р— D — Q
Transcribed Image Text:E1 E2 ЕЗ E4 E5 X— Ү — Z — Р— D — Q
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