You have treated undifferentiated embryonic stem cells (ESCs) with sonic hedgehog (Shh) for several days. This should induce a neural fate in your ESCs and you hope that you have been successful in differentiating the ESCs into NSCs. What is one assay you could use to test whether your cells had differentiated into NSC-like cells? Name and shortly describe the assay?

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You have treated undifferentiated embryonic stem cells (ESCs) with sonic hedgehog (Shh)
for several days. This should induce a neural fate in your ESCs and you hope that you have
been successful in differentiating the ESCs into NSCs. What is one assay you could use to test
whether your cells had differentiated into NSC-like cells? Name and shortly describe the assay?

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