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You have isolated a protein from the bacterium E. coli and seek to confirm its identity by trypsin digestion and mass spectrometry. Determination of the masses of several peptide fragments has enabled you to deduce the identity of the protein. However, there is a discrepancy with one of the peptide fragments, which you believe should have the sequence MLNSFK and an (M + H)+ value of 739.38. In your experiments, you repeatedly obtain an (M + H)* value of 767.38. What is the cause of this discrepancy? The peptide is phosphorylated. The peptide is formylated. The peptide has an additional residue. The peptide is hydroxylated. What does this discrepancy tell you about the region of the protein from which this peptide is derived? This peptide corresponds to the most C-terminal fragment of the protein. This peptide corresponds to an internal region of the protein. This peptide corresponds to the most N-terminal fragment of the protein. The region of the protein from which this peptide is derived cannot be determined.

You have isolated a protein from the bacterium E. coli and seek to confirm its identity by trypsin digestion and mass spectrometry. Determination of the masses of several peptide fragments has enabled you to deduce the identity of the protein. However, there is a discrepancy with one of the peptide fragments, which you believe should have the sequence MLNSFK and an (M + H)+ value of 739.38. In your experiments, you repeatedly obtain an (M + H)* value of 767.38. What is the cause of this discrepancy? The peptide is phosphorylated. The peptide is formylated. The peptide has an additional residue. The peptide is hydroxylated. What does this discrepancy tell you about the region of the protein from which this peptide is derived? This peptide corresponds to the most C-terminal fragment of the protein. This peptide corresponds to an internal region of the protein. This peptide corresponds to the most N-terminal fragment of the protein. The region of the protein from which this peptide is derived cannot be determined.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You have isolated a protein from the bacterium E. coli and seek to confirm its identity by trypsin digestion and mass spectrometry. Determination of the masses of several peptide fragments has enabled you to deduce the identity of the protein. However, there is a discrepancy with one of the peptide fragments, which you believe should have the sequence MLNSFK and an (M + H)* value of 739.38. In your experiments, you repeatedly obtain an (M + H)* value of 767.38. + What is the cause of this discrepancy? O The peptide is phosphorylated. The peptide is formylated. The peptide has an additional residue. The peptide is hydroxylated. What does this discrepancy tell you about the region of the protein from which this peptide is derived? This peptide corresponds to the most C-terminal fragment of the protein. This peptide corresponds to an internal region of the protein. This peptide corresponds to the most N-terminal fragment of the protein. The region of the protein from which this peptide is derived cannot be determined.
Transcribed Image Text:You have isolated a protein from the bacterium E. coli and seek to confirm its identity by trypsin digestion and mass spectrometry. Determination of the masses of several peptide fragments has enabled you to deduce the identity of the protein. However, there is a discrepancy with one of the peptide fragments, which you believe should have the sequence MLNSFK and an (M + H)* value of 739.38. In your experiments, you repeatedly obtain an (M + H)* value of 767.38. + What is the cause of this discrepancy? O The peptide is phosphorylated. The peptide is formylated. The peptide has an additional residue. The peptide is hydroxylated. What does this discrepancy tell you about the region of the protein from which this peptide is derived? This peptide corresponds to the most C-terminal fragment of the protein. This peptide corresponds to an internal region of the protein. This peptide corresponds to the most N-terminal fragment of the protein. The region of the protein from which this peptide is derived cannot be determined.
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