You have a 1.270 ug/uL DNA stock. What volume will give you 10 ug?

Q: Please convert to past tense and passive voice by using point form

A: The experimental procedure contains the specific techniques used in conducting a particular…

Q: absorbance (A260) OD1 , in actual measurements, Does dsDNA's concentration always come up as…

A: The yield of DNA can be determined by optical density or absorbance. The most common readings…

Q: In this gel, which letter (A. B. or C) indicates the largest piece of DNA in lane one? If the…

A: Gel electrophoresis is a laboratory technique used to separate macromolecules such as DNA, RNA and…

Q: Does each sample contain pure DNA? How do you know?

A: Not necessarily all the samples will have pure DNA it may have contaminations and the level of…

Q: 1b) When doing automated sequencing, all 4 dideoxynucleotides are added to the same sequencing…

A: DNA sequencing is a biochemical method for determining the order of the nucleotide bases, adenine,…

Q: DNA Standard Markers A --- Control DNA 4282 Patient Peripheral Blood DNA 4282 3000 1282 D Patient…

A: Blood From peripheral, tumor and breast samples all have its own genetic identity or fingerprint…

Q: The letter R isconventionally used to represent anypurine base (A or G) and Y to represent…

A: Purines and pyrimidines are the two families of nitrogeneous base that are used to make nucleic…

Q: researcher runs an agarose gel IIl ladder one läñé ând 100 ng of a 500 bp DNA fragment in another…

A: Agarose gel electrophoresis or AGE is a form of gel electrophoresis that is used for discriminate…

Q: A typical STR DNA type emanating from a single individual shows a (one, two, three)-band pattern.

A: STR stands for Short tandem repeats, which are useful for forensic scientists because they enable…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: Lane 1 bottom band has below 500 basepairs. Lane 1 top band has 5000bp. Lane 2 band 1000bp.

Q: Estimate the fragment sizes (bp) of the DNA bands from each sample lane

A: Introduction: In the gel electrophoresis, agarose derived from red seaweed acts as a sieve for…

Q: 41) In a bacterial cross in which the donor (Hfr) is a+b+ and the recipient strain (F-) is a-b-, it…

A: D) Will all be a+b+ Explain;- The formation can be characterized as the course of transmission of…

Q: You have 15 tubes, each containing 5 ul of DNA (each tube has a different DNA to cut). You want to…

A: A reaction mixture is a combination of 2 or more reactants in definite proportions to carry out a…

Q: Identify the solution component that contains the extracted DNA.

A: Ethanol precipitation method is generally used for DNA extraction.

Q: Four E. coli strains of genotype atb¯ are labeled 1, 2, 3, and 4. Four strains of genotype a¯b* are…

A:

Q: Mother = 2) Child ||| 16. 'Dad' 1 'Dad' 2 || 9) FAC 9) 'Dad' 3 Source:…

A: DNA fingerprinting or profiling refers to any DNA-based technique that identifies DNA from a…

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: How much 6x loading dye should be added to 49 uL of DNA?  Report you answer in units of uL, with one…

A: The equation of dilution: M1xV1 = M2xV2 Where, M1 is the molar concentration of the stock solution.…

Q: You have run a gel with 5 uL of amplified DNA in addition to 1 uL of 6X loading dye. Comparing the…

A: Agarose Gel Electrophoresis (AGE), is an biochemistry technique which is used to estimate DNA…

Q: Why was there a greater A260 absorbance reading for your DNA sample that was incubated at higher…

A: Hyperchromicity is the increase in optical density of a substance at a particular wavelength.

Q: Base pairing can be found between two purines in DNA.   True   False

A: We all know that DNA and RNA can act as nucleic acids. These DNA and RNA are the polymer of…

Q: A 100ml dsDNA solution prepared via 1:20 dilution was taken for spectrometry analysis. The A260 and…

A: The objective of the question is to calculate the total DNA yield of the solution in micrograms. The…

Q: How much 4x loading dye should be added to 11 uL of DNA?  Report you answer in units of uL, with one…

A: Recall the equation of dilution: C1 x V1 = C2 x V2 Where, C1 is the concentration of the stock…

Q: You digest 4 uL of plasmid DNA that is 50 ng/uL concetration in a total volume of 20 uL. You run 10…

A: Plasmid DNA is a small, circular, double-stranded DNA molecule that is commonly found in bacteria…

Q: d)Halfway through the electrophoresis of your gel you realized that rather than using a buffer to…

A: D) As we know, the distilled water is neutral i.e. it doesn't contain any ions. Thus, when halfway…

Q: You are given two samples of DNA, one from Clostridium perfringens and the other from Mycobacterium…

A: DNA is a nucleic acid that is the genetic material for most organisms (except some virus). It…

Q: How many uL of DNA would I load into my gel if I am given a stock of 450ug/mL and I want to load…

A: Introduction :- DNA, or Deoxyribonucleic Acid, is a long, double-stranded molecule that carries the…

Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.