You find in the literature that an enzyme purified very close to homogeneity has a specific activity of 12,300 U/mg. What is likely to be the largest source of error in the quoted value? U

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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You find in the literature that an enzyme purified very close to homogeneity has a specific
activity of 12,300 U/mg. What is likely to be the largest source of error in the quoted value? U
(unit) typically is defined as the amount of enzyme that is required to consume 1.00 umoles of
substrate per minute under some given set of conditions. Units are typically determined
spectrophotometrically by measuring the rate of formation of product.
Transcribed Image Text:You find in the literature that an enzyme purified very close to homogeneity has a specific activity of 12,300 U/mg. What is likely to be the largest source of error in the quoted value? U (unit) typically is defined as the amount of enzyme that is required to consume 1.00 umoles of substrate per minute under some given set of conditions. Units are typically determined spectrophotometrically by measuring the rate of formation of product.
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