You discovered a novel enzyme URase that you suspect to be involved in sugar metabolism. You have already found in vitro assay conditions for accurate measurements of enzyme activity. However, you do not know its physiological substrate. Since the microorganism from which the enzyme was discovered thrives on fruits, it could be fructose. Or, it could be glucose. You will carry out kinetic experiments to determine which sugar molecule is the likely physiological the substrate of URase. Will you determine some kinetic parameters? What experiments will do you to determine those parameters? Provide experimental procedures without going into details, and how will you decide which sugar is the likely substrate? Explain your rationale.
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
You discovered a novel enzyme URase that you suspect to be involved in sugar
already found in vitro assay conditions for accurate measurements of enzyme activity. However, you
do not know its physiological substrate. Since the microorganism from which the enzyme was
discovered thrives on fruits, it could be fructose. Or, it could be glucose.
You will carry out kinetic experiments to determine which sugar molecule is the likely physiological
the substrate of URase.
Will you determine some kinetic parameters? What experiments will do you to determine those parameters? Provide experimental procedures without going into details, and how will you decide which sugar is the likely substrate? Explain your rationale.
Step by step
Solved in 3 steps
