You think you need may have found a new inhibitor (NI) of the HIV protease, so you performed few experiments to characterize its behavior. You found that if you add an excess of substrate to the protease in the presence of the inhibitor, enzyme activity decreased at low substrate but reached the same vmax as observed in the absence of the inhibitor. Also, the affinity of the substrate for the enzyme is decreased by the inhibitor. What class of inhibitor is NI, competitive, uncompetitive, non competitive, or mixed type?
You think you need may have found a new inhibitor (NI) of the HIV protease, so you performed few experiments to characterize its behavior. You found that if you add an excess of substrate to the protease in the presence of the inhibitor, enzyme activity decreased at low substrate but reached the same vmax as observed in the absence of the inhibitor. Also, the affinity of the substrate for the enzyme is decreased by the inhibitor. What class of inhibitor is NI, competitive, uncompetitive, non competitive, or mixed type?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
You think you need may have found a new inhibitor (NI) of the HIV protease, so you performed few experiments to characterize its behavior. You found that if you add an excess of substrate to the protease in the presence of the inhibitor, enzyme activity decreased at low substrate but reached the same vmax as observed in the absence of the inhibitor. Also, the affinity of the substrate for the enzyme is decreased by the inhibitor. What class of inhibitor is NI, competitive, uncompetitive, non competitive, or mixed type?
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 3 steps
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON