You design primers that will amplify a 400 bp fragment of the CAT gene and perform PCR. The 400 bp CAT fragment is shown below. The locations where the designed primers anneal are bolded and underlined. 5'GGATTTGGTCGTATTAGGCCTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTCAGACACCATGGGGAAGTG AAGGTCGGAGTCAACGGATTTGGTCGTTTCTTTTGCGTCGCCAGCCGAGCCACATCGCTCAGACACATGGGGA AGTGAAGGTCGGAGTCAACACACTGGGTCATCCCGTCGCGCCTGGTCACCAGGGCTGCTTTTAACTCTGTTCG ACAGTCAGCCGCATCGGAAAGTGGATATTGTTGCCATCAATGACATTGGGCGCCTGGTCACCAGGGCTGCTTT ТААСТСТGGAAAGTGGATAТTGTTGCCATСААTGACCCOТТСАТТGACСТСААСТАСАTGGTTTACATGTTC AATAGATTCCACCCATGAGCCGAGCCACATCGCTCAG -3' a. Write the forward primer sequence, indicate 5' and 3' ends b. Write the reverse primer sequence, indicate 5' and 3' ends

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Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.