3.You are studying an enzyme in the laboratory that is only active between pH 5 and 8.You wish to study the activity in a buffer of pH 7. Pick the best buffer system formaking your buffer:Buffer systemTris/HCIAcetic acid/acetateLactic acid/lactateCarbonateBicarbonatePhosphatepka8.14.83.96.410.27.2

A buffer solution is a water solvent-based solution consisting of mixture of weak acid and its…

Answered: You are studying an enzyme in the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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(8). Na2EDTA is actually used in the lab because ____ a. it does not lose its crystalline water component. it loses its crystalline water component thus makes a constant composition. it is a base which is needed to react with metal ions. it is easier to be dissolved in water than EDTA. it is only commercially available EDTA-related reagent. (9). Which of the following statement is true ? ____ a. EDTA is an amino acid. Usually we use the acidic form of EDTA for the lab use. EDTA stands for “ Ethyldimertetraacid” EDTA forms complexes with metal ions in a variable stoichiometry. EDTA usually offers 5 binding sites for metal ions. None of the above (10). “The determination of CuO ore sample” lab is a ____ a. Acid-base titration b. complexometric titration c. iodometry d. gravimetry e. iodimetry
Please discuss the buffering capacity of an amino acid. In your answer include what is a buffer? Why is it important? How can amino acids act as buffers? A▾ B I X 7
What is the initial velocity measurement for the hydrolysis of 50 μM amoxicillin by the enzyme?
I need to calculate the total change in pH
You should change it to Catechol (ml) 0.2 0.2 0.2 0.2 ml Turnip extract (ml) 0.1 0.1 0.2 Buffer (ml) 1 2 3 4 H₂O₂ (ml) Distilled H2O (ml) 1.7 1.2 1.1 1.1 Total Volume 0.5 0.5 0.5 0.5 0.5 pH 7 pH 7 pH 7 pH 7 0 0.5 0.5 0.5 2.5 2.5 2.5 2.8
How do I calculate the final concentration? I just need one solution to walk through the steps. Prepare eight standards of glucose with your 1 mM glucose solution, each with a final volume of 10 mL. Complete the table on the next page, indicating what your final concentration of glucose will be for each standard, in mM. Volume of Potassium Ferrocyanide Solution Volume of Enzyme Solution Volume of 1 mM glucose solution Final volume of solution Final Concentration of glucose in final solution (mM) 2.50 mL 1.00 mL 0.00 mL 10.00 mL 2.50 mL 1.00 mL 0.50 mL 10.00 mL 2.50 mL 1.00 mL 1.00 mL 10.00 mL 2.50 mL 1.00 mL 2.00 mL 10.00 mL 2.50 mL 1.00 mL 3.00 mL 10.00 mL 2.50 mL 1.00 mL 4.00 mL 10.00 mL 2.50 mL 1.00 mL 5.00 mL 10.00 mL 2.50 mL 1.00 mL 6.00 mL 10.00 mL
If there is an increase in the hydrogen ion concentration from 38 nmol.l-1 to 82 nmol.l-1, what does the pH change from and to?
A scientist extracted compounds from apple peel and separated them using gel exclusion chromatography. Indicate which would be the expected order of elution from first eluted to last.
The pH scale shows availability of reactive hydrogen ions (H+) in a liquid. The scale is logarithmic, so milk has 10 times as many H+ ions as pure water, for a given volume. How many more H+ ions does soda pop have compared to pure water? Write the number only.
Many manufacturers claim that their health care and hair care products are pH balanced or buffered. How would you test their claims?
The pH of a solution is 7, what is the H+ concentration? Group of answer choices 1 x 10e-7 M 1 x 10e-3 M 1 x 10e-8 M 1 x 10e-9 M
Which of the following statements about pH is (are) true: pH is a measure of free hydrogen ion concentration none of these are true both of these are true An increase in hydrogen ion concentration means a decrease in pH scale units

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