You are setting up an experiment to stain cells with DAPI (4',6-diamidino-2-phenylindole). Your stock solution of DAPI is 1 mg/mL. You need your finalstaining solution to be 1 ug/mL.a) What is the dilution factor you need to use to go from your stock solution (1mg/mL) to your final staining solution (1 ug/mL)?you can.Show your workings if.b) To what regions of DNA does DAPI bind? Describe in terms of the bases andpart of the DNA double helix.c) You notice multiple especially dark speckles in the nuclei of your cells whenyou look under a microscope. What kind of DNA/chromatin do you think this is?Think in terms of more or less actively transcribed DNA regions.

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You are setting up an experiment to stain cells with DAPI (4',6-diamidino-2- phenylindole). Your stock solution of DAPI is 1 mg/mL. You need your final staining solution to be 1 ug/mL. a) What is the dilution factor you need to use to go from your stock solution (1 mg/mL) to your final staining solution (1 ug/mL)? Show your workings if you can. b) To what regions of DNA does DAPI bind? Describe in terms of the bases and part of the DNA double helix. c) You notice multiple especially dark speckles in the nuclei of your cells when you look under a microscope. What kind of DNA/chromatin do you think this is? Think in terms of more or less actively transcribed DNA regions.

You are setting up an experiment to stain cells with DAPI (4',6-diamidino-2- phenylindole). Your stock solution of DAPI is 1 mg/mL. You need your final staining solution to be 1 ug/mL. a) What is the dilution factor you need to use to go from your stock solution (1 mg/mL) to your final staining solution (1 ug/mL)? Show your workings if you can. b) To what regions of DNA does DAPI bind? Describe in terms of the bases and part of the DNA double helix. c) You notice multiple especially dark speckles in the nuclei of your cells when you look under a microscope. What kind of DNA/chromatin do you think this is? Think in terms of more or less actively transcribed DNA regions.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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