**TLC Experiment Improvement Suggestions****Problem Statement:**A student gets the following results after running a TLC plate of several over-the-counter analgesics. What should the student do to get better results?**Diagram Explanation:**The diagram depicts a TLC (Thin Layer Chromatography) plate with a baseline featuring five dots. All dots remain at the baseline, suggesting minimal or no movement of the sample spots up the plate.**Options for Improvement:**- **Run the TLC for a longer time.** - Extending the duration might allow compounds more time to separate and move further up the plate.- **Co-spot the sample several times.** - Applying multiple layers of the sample might increase the concentration, though if movement isn't occurring, this will not improve results.- **Run a new TLC plate in a less polar solvent.** - A less polar solvent might lead to different separation behaviors, but if the compounds are too polar themselves, they may not move much.- **Run a new TLC in a more polar solvent mixture.** - Using a more polar solvent could help the compounds move further if they have strong interactions with polar solvents. This is often an effective first strategy.**Recommendation:**The most plausible option to achieve better separation, based on the chart, would be to run a new TLC plate in a more polar solvent mixture. This might facilitate the movement of compounds from the baseline.

If we get above results after running a TLC plate several over the counter analysis , for better…

Answered: 7. A student gets the following results…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Similar questions

Based on the TLC results label spots 1,2,3 with aspirin, acetaminophen, and caffein. Make sure to give the Rf value for each.
Your HRP sample had a concentration of 2.0 mg/mL and you added 10 uL to a tube containing 10 uL of 2X Sample Buffer. You mixed this well and then heated at 75C for 5 min. Then you added 10 uL of this solution to a well of a gel. How much protein did you add to that well?
Sample #1 has an absorbance of 0.300. Sample #2 is the same substance and has an absorbance of 0.600 using the same equipment. What is the relative concentration of the second sample? Select one: a. The concentration of sample #2 is the square root of that of #1. b. The concentration of sample #2 is that of sample #1 squared. c. The concentration of sample #2 is half that of sample #1. d. The concentration of sample #2 is twice that of sample #1.
What, if any, impact is using drops instead of specific volumes has on the experiment?
TLC relies on differences in non-covalent forces. Explain the role of these non-covalent interactions in determining the Rf value obtained.
There were 2 spots on the TLC that did not match up with any of the standards. What were the Rf values of these spots? Identify what these chemical species might be and draw their structures. Solvent was 3:1:1 n-butanol/acetic acid/water. First plate is TLC plate 1. Second plate is TLC plate 2.
What is being described in the following items? ✓light that interacts with the solution ✓light not absorbed by the solution gives relative amount of analyte in the solution I I A. transmitted light B. absorbance C. incident light D. pathlength
4
We used tab water to extract caffeine from tea instead of using distilled water. How does it affect your result? Explain it scientifically. If you forgot to add anhydrous sodium sulfate, how does it affect your result? Explain it scientifically If you forgot to cap the TLC chamber, how does it affect your result? Explain it scientifically.
Running an HPLC assay using a column heated to approximately 60 °C can have what benefits over running the assay room temperature? Select all that are true. ✔increased column life decreased run time greatly enhanced peak shape ✔ more precise quantitation O flucuating retention times O increased back pressure more precise retention times Submit Answer Incorrect. Tries 1/4 Previous Tries
Identify the most polar substance shown on the following TLC plate. Yellow spot Blue spot Red spot
A student developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown in the figure. Determine the retention factor (Rf) values of each spot on dye C. (use 2 decimal places) What other dye/s are present in dye C and what is the dye with least number of pigments?

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