Problem 4: You are planning an experiment to use zinc finger nucleases to generate a double-stranded DNA break in a targeted sequence of DNAName the protein domain in a zinc finger nuclease that is responsible for generating the DNA breakFok1Name two other genome-engineering proteins that could be used for generating the double-stranded DNA break What are the advantages and disadvantages of each strategy? Problem 4:You are planning an experiment to use zinc finger nucleases to generate a double-strandedDNA break in a targeted sequence of DNA(a) Name the protein domain in a zinc finger nuclease that is responsible for generating theDNA break(b) Name two other genome-engineering proteins that could be used for generating thedouble-stranded DNA break(c) What are the advantages and disadvantages of each strategy?

Zinc finger nucleases (ZFNs) are synthetic DNA restriction enzymes that are made up of FokI…

You are planning an experiment to use zinc finger nucleases to generate a double-stranded DNA break in a targeted sequence of DNA Name the protein domain in a zinc finger nuclease that is responsible for generating the DNA break Fok1 Name two other genome-engineering proteins that could be used for generating the double-stranded DNA break What are the advantages and disadvantages of each strategy?

You are planning an experiment to use zinc finger nucleases to generate a double-stranded DNA break in a targeted sequence of DNA Name the protein domain in a zinc finger nuclease that is responsible for generating the DNA break Fok1 Name two other genome-engineering proteins that could be used for generating the double-stranded DNA break What are the advantages and disadvantages of each strategy?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 12TYK

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Concept explainers

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.

Question

Problem 4:

You are planning an experiment to use zinc finger nucleases to generate a double-stranded DNA break in a targeted sequence of DNA

Name the protein domain in a zinc finger nuclease that is responsible for generating the DNA break
1. Fok1
Name two other genome-engineering proteins that could be used for generating the double-stranded DNA break
What are the advantages and disadvantages of each strategy?

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