Which type of stain would be the best choice for negative staining of most bacterial cells: a basic stain or an acidic stain? Explain your answer.
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Which type of stain would be the best choice for negative staining of most bacterial cells: a basic stain or an acidic stain? Explain your answer.
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- Which type of stain would be the best choice for negative staining of most bacterial cells: a basic stain or an acidic stain?What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIUMethylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect the staining of bacteria? Can dyes other than methylene blue be used for direct staining? Briefly explain.
- Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.Is it possible to perform double or triple staining using fluorescent dyes? Explain.Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?
- Is acid fast staining differential stain , if yes, what are the 2 types of stain usedDraw what you would expect to see for acid fast stain and endospore stain procedure if you did not stain with counterstain. Color pencils need to be used.a: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.
- What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.Why are basic dyes more effective for bacterial staining than acidic dyes?Based from the results obtained from Gram staining, explain why bacterial cells retained or changed their color. Briefly explain the principle behind the procedure. Cite advantages of differential staining method (Gram staining and endospore staining) over simple staining only. List other staining methods aside from those you performed in the laboratory. conclusion: