Why do acidic dyes not stain bacterial cells?

Q: After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in…

A: Introduction Gram staining, commonly known as Gram's method, is a staining technique that is used to…

Q: Would pigment production by the bacteria affect absorbance reading?

A: Bacteria produces various types of pigments.

Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…

A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…

Q: Please discuss why staining is important in visualizing microbes under the microscope. What…

A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…

Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…

A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…

Q: NMR is considered a low-sensitivity spectroscopic technique. What is the physical basis of NMR's…

A: Nuclear magnetic resonance is a natural phenomenon during which nuclei during a strong constant…

Q: 1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2)…

A: The staining techniques are used to identify the morphological characteristics along with the size…

Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…

A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…

Q: What is the purpose of using a biological stain when microscopically examining cellular components?

A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…

Q: Can colorless samples be analyzed using a UV-Vis spectrophotometer? Yes or No or Maybe?

A: UV-Vis Spectroscopy  It is defined as a quantitative technique that is used to identify the…

Q: What do you understand from negative stain, simple stain, and acid-fast stain?

A: Negative stains, simple stains, and acid-fast stains are all techniques used in microbiology to…

Q: Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain…

A: 1.Since the bacteria are negatively charged, a positive charge was needed to cause staining. The…

Q: why steroids do not show luminescence on thin layer chromatographic plates?

A: Infrared absorption spectroscopy also stated as IR spectroscopy is one of the most valuable…

Q: Why isn’t the Gram stain used on acid-fast bacteria? If you did Gram stain acid-fast bacteria, what…

A: Gram stain cannot be used on acid-fast bacteria because acid-fast staining is done on mycobacterium…

Q: Which form of the Coomassie dye do you expect to be more prevalent in a low pH solution? Group of…

A: Coomassie dye binds to proteins through ionic interactions between sulfonic acid groups and…

Q: Define dyes and describe the basic chemistry behind the processof staining.

A: Detection and observation of cells and microorganisms in their natural state under microscope is…

Q: What is the difference between Raman Spectroscopy and SERS ( Surface Enhanced Raman Spectroscopy).

A: Introduction: It is the most powerful tool to study the interactions between matter and…

Q: Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not…

A: only acid fast appears red/pink. Only endospore appears green. Vegetative cell become colourless.

Q: During staining, the first step is to dewax the slide. What is the purpose of the step? What is the…

A: Introduction :- In microscopy, staining is a technique used to increase contrast in a microscopic…

Q: commonly used to stain cytoplasm granules blue

A: On the basis of staining the microbes can also be distinguished as gram-positive and gram-negative…

Q: For What purpose  is Giemsa stain used for?

A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…

Q: Why do we stain some organisms before viewing them under a microscope? (see top of p 14)

A: Microbiology is the study of microorganism. The organisms that cannot be seen through naked eyes are…

Q: How do stains used for light microscopy compare withthose used for electron microscopy?

A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…

Bacterial Morphology

The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology. 

Bacterial cell structure

Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.