When answering the questions below, please use the ONE-LETTER CODE for the amino acid, with NO spaces and symbols between each letter. a. What is the C-terminal residue?  b. What is the N-terminal residue?  c. What is the sequence of Peptide 2?  d. What is the sequence of Peptide 4?  e. What is the overall amino acid sequence?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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When answering the questions below, please use the ONE-LETTER CODE for the amino acid, with NO spaces and symbols between each letter.
a. What is the C-terminal residue? 
b. What is the N-terminal residue? 
c. What is the sequence of Peptide 2? 
d. What is the sequence of Peptide 4? 
e. What is the overall amino acid sequence? 

A HEPTAPEPTIDE that punctures the bacterial cell wall has just been recently isolated from the venom gland of a frog. Amino acid analysis of the peptide reveals the following information
below: (NOTE: when the sequence is not known, a comma separates the amino acids)
Hydrozine
Acid Hydrolysis (6 N HCI) at 110 °C
Heptapeptide
(2) Arg, Pro, Phe, Tyr, Ala, Lys
2,4-dinitrofluorobenzene
DNP-Phe
Lys and
modified free
Peptide 1
(Ala, Lys)
amino acids
Cleavoge with Trypsin, then chromatogrophy
Peptide 2
(Pro, Arg, Tyr)
Peptide 3
(Phe, Arg)
Phe
| Cleavage with Chymotrypsin, then chromatography
Peptide 4
(Arg, Tyr)
Peptide 5
(Lys, Pro, Ala, Arg)
Transcribed Image Text:A HEPTAPEPTIDE that punctures the bacterial cell wall has just been recently isolated from the venom gland of a frog. Amino acid analysis of the peptide reveals the following information below: (NOTE: when the sequence is not known, a comma separates the amino acids) Hydrozine Acid Hydrolysis (6 N HCI) at 110 °C Heptapeptide (2) Arg, Pro, Phe, Tyr, Ala, Lys 2,4-dinitrofluorobenzene DNP-Phe Lys and modified free Peptide 1 (Ala, Lys) amino acids Cleavoge with Trypsin, then chromatogrophy Peptide 2 (Pro, Arg, Tyr) Peptide 3 (Phe, Arg) Phe | Cleavage with Chymotrypsin, then chromatography Peptide 4 (Arg, Tyr) Peptide 5 (Lys, Pro, Ala, Arg)
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