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Predict the amino acid sequence based on the conditions below. Use the one-letter abbreviation of amino acids.

Predict the amino acid sequence based on the conditions below. Use the one-letter abbreviation of amino acids.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Predict the amino acid sequence based on the conditions below. Use the one-letter abbreviation of amino acids. ___1___--_2_- 3 -- --____6_--_7__ a. The amino acid in the C-terminal is optically inactive. b. The fifth amino acid is a neutral aromatic amino acid that yields a negative result for the Xanthoproteic test using intact protein but produces a yellow soln with basic hydrolysate. It also gives a negative result with both Hopkin's Cole and Millon's test using basic hydrolysate. c. After treatment of this peptide with aminopeptidase (specificity: peptide bond on the N-terminus), it yields serine and a hexapeptide d. The amino acid besides the N-terminal amino acid. will yield ornithine and urea after alkaline hydrolysis. e. The third amino acid gives a positive result for Hopkins-Cole Test. f. Chymotrypsin^ cannot cleave the peptide bond on the fifth amino acid because of the presence of this a.a. residue. g. The amino acid at the carboxy-end of the third amino acid will yield a black precipitate with Pb(Ac)2 reagent. *Chymotrypsin is an enzyme that cleaves the peptide bond at the carboxyl end of an aromatic amino acid except when proline is bonded to it
Transcribed Image Text:Predict the amino acid sequence based on the conditions below. Use the one-letter abbreviation of amino acids. ___1___--_2_- 3 -- --____6_--_7__ a. The amino acid in the C-terminal is optically inactive. b. The fifth amino acid is a neutral aromatic amino acid that yields a negative result for the Xanthoproteic test using intact protein but produces a yellow soln with basic hydrolysate. It also gives a negative result with both Hopkin's Cole and Millon's test using basic hydrolysate. c. After treatment of this peptide with aminopeptidase (specificity: peptide bond on the N-terminus), it yields serine and a hexapeptide d. The amino acid besides the N-terminal amino acid. will yield ornithine and urea after alkaline hydrolysis. e. The third amino acid gives a positive result for Hopkins-Cole Test. f. Chymotrypsin^ cannot cleave the peptide bond on the fifth amino acid because of the presence of this a.a. residue. g. The amino acid at the carboxy-end of the third amino acid will yield a black precipitate with Pb(Ac)2 reagent. *Chymotrypsin is an enzyme that cleaves the peptide bond at the carboxyl end of an aromatic amino acid except when proline is bonded to it
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